Center for Research and Advanced Studies of the IPN, Irapuato Unit, Genetic Engineering Department, Irapuato 36824, Mexico.
Int J Mol Sci. 2021 Mar 17;22(6):3068. doi: 10.3390/ijms22063068.
RNA-binding proteins (RBPs) are key elements involved in post-transcriptional regulation. Ataxin-2 (ATXN2) is an evolutionarily conserved RBP protein, whose function has been studied in several model organisms, from to the . ATXN2 interacts with poly(A) binding proteins (PABP) and binds to specific sequences at the 3'UTR of target mRNAs to stabilize them. () and are two ATXN2 orthologs present in plant genomes whose function is unknown. In the present study, phenotypical and transcriptome profiling were used to examine the role of and in . We found that they act redundantly to influence pathways throughout the life cycle. cid3cid4 double mutant showed a delay in flowering time and a reduced rosette size. Transcriptome profiling revealed that key factors that promote floral transition and floral meristem identity were downregulated in whereas the flowering repressor () was upregulated. Expression of key factors in the photoperiodic regulation of flowering and circadian clock pathways, were also altered in , as well as the expression of several transcription factors and miRNAs encoding genes involved in leaf growth dynamics. These findings reveal that orthologs may have a role in developmental pathways throughout the life cycle of plants.
RNA 结合蛋白 (RBPs) 是参与转录后调控的关键元件。Ataxin-2 (ATXN2) 是一种进化上保守的 RBP 蛋白，其功能已在几种模式生物中进行了研究，从 到 。ATXN2 与 poly(A) 结合蛋白 (PABP) 相互作用，并与靶 mRNA 的 3'UTR 上的特定序列结合以稳定它们。() 和 是植物基因组中存在的两个 ATXN2 同源物，其功能未知。在本研究中，使用表型和转录组谱分析来研究 和 在 中的作用。我们发现它们冗余地作用以影响整个生命周期中的途径。cid3cid4 双突变体表现出开花时间延迟和莲座叶尺寸减小。转录组谱分析显示，促进花转变和花分生组织身份的关键因子在 中下调，而开花抑制因子 () 上调。参与光周期调控开花和生物钟途径的关键因子的表达也在 中发生改变，以及涉及叶生长动态的几个转录因子和 miRNA 编码基因的表达。这些发现表明 同源物可能在植物整个生命周期的发育途径中发挥作用。
