Insect Physiology, Molecular Biology and Microbiology Agricultural Research and Extension Trust, Private Bag 9, Lilongwe, Malawi.
Division of Entomology, Indian Agricultural Research Institute, New Delhi, 110012, India.
Sci Rep. 2024 Sep 27;14(1):22400. doi: 10.1038/s41598-024-73417-5.
This study utilized cultivable methods and 16 S amplicon sequencing to compare taxonomic profiles and functional potential of gut bacteria in the scarab beetle, Anomola dimidiata, under cellulose-enriched conditions. Eight culturable cellulolytic gut bacteria were isolated from the midgut and hindgut of the scarab larvae, respectively. 16 S amplicon sequencing evinced that the most represented taxonomic profiles at phylum level in the fermentation chamber and midgut were Bacillota (71.62 and 56.76%), Pseudomonadota (22.66 and 36.89%) and Bacteroidota (2.7 and 2.81%). Bacillota (56.74 and 91.39%) were significantly enriched in the midgut with the addition of cellulose. In contrast, Bacillota and Psedomonadota were significantly enriched in the fermentation chamber. Carbohydrate metabolism was up-regulated in the midgut, while nitrogen and phosphorus metabolism were up-regulated in the fermentation chamber, suggesting these symbionts' possible metabolic roles to the host. An analysis of total cellulases as well as amplicon sequence variants indicated that the gut bacteria belonging to Acinetobacter, Bacillus, Brucella, Brevibacillus, Enterobacter, Lysinibacillus and Paenibacillus are involved in nutrition provisioning. These results have provided additional insights into the gut bacteria associated with cellulose digestion in A. dimidiata and created a platform for bioprospecting novel isolates to produce biomolecules for biotechnological use, besides identifying eco-friendly targets for its management.
本研究利用可培养方法和 16S 扩增子测序技术，比较了在富含纤维素的条件下，金龟子幼虫肠道细菌的分类组成和功能潜力。从金龟子幼虫的中肠和后肠分别分离出 8 株可培养的纤维素分解肠道细菌。16S 扩增子测序结果表明，在发酵室和中肠中，门水平上最主要的分类组成分别为芽孢杆菌门（71.62%和 56.76%）、假单胞菌门（22.66%和 36.89%）和拟杆菌门（2.7%和 2.81%）。添加纤维素后，中肠中芽孢杆菌门（56.74%和 91.39%）显著富集。相比之下，芽孢杆菌门和假单胞菌门在发酵室中显著富集。碳水化合物代谢在中肠中上调，而氮磷代谢在发酵室中上调，这表明这些共生菌可能对宿主有代谢作用。对总纤维素酶和扩增子序列变异的分析表明，属于不动杆菌属、芽孢杆菌属、布鲁氏菌属、短芽孢杆菌属、肠杆菌属、赖氨酸芽孢杆菌属和类芽孢杆菌属的肠道细菌参与了营养供应。这些结果为金龟子幼虫与纤维素消化相关的肠道细菌提供了更多的信息，并为生物勘探新型分离物以生产生物技术用生物分子创造了一个平台，同时还确定了其管理的环保目标。
