Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150007, China.
Central Laboratory of The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.
J Transl Med. 2024 Sep 27;22(1):857. doi: 10.1186/s12967-024-05667-w.
In this study, we dynamically monitored the glomerular filtration rate and other assessment of renal function and markers of injury in various mice models of acute kidney injury. Male C57BL/6 mice were utilized to establish acute kidney injury models of sepsis, ischemia reperfusion, cisplatin, folic acid, aristolochic acid and antibiotic. In addition to the real time glomerular filtration rate, renal LCN-2 and HAVCR-1 mRNA expression levels, and serum creatinine, urea nitrogen and cystatin c levels were also used to evaluate renal function. In addition, the protein levels of LCN-2 and HAVCR-1 in renal, serum and urine were measured. Our results demonstrated that the changes in biomarkers always lagged the real time glomerular filtration rate during the progression and recovery of renal injury. Cystatin-c can reflect renal injury earlier than other markers, but it remains higher in the recovery stage. Perhaps the glomerular filtration rate does not reflect the greater injury caused by vancomycin plus piperacillin.
在这项研究中，我们动态监测了各种急性肾损伤小鼠模型的肾小球滤过率和肾功能及损伤标志物。雄性 C57BL/6 小鼠被用于建立脓毒症、缺血再灌注、顺铂、叶酸、马兜铃酸和抗生素所致急性肾损伤模型。除了实时肾小球滤过率外，还利用肾 LCN-2 和 HAVCR-1mRNA 表达水平以及血清肌酐、尿素氮和胱抑素 C 水平来评估肾功能。此外，还测量了肾、血清和尿中 LCN-2 和 HAVCR-1 的蛋白水平。我们的结果表明，在肾损伤的进展和恢复过程中，生物标志物的变化总是滞后于实时肾小球滤过率。胱抑素-c 比其他标志物更早地反映肾损伤，但在恢复阶段仍较高。也许肾小球滤过率并不能反映万古霉素加哌拉西林引起的更大损伤。
