Schlegel W, Kusseler R, Nieschlag E
Arch Androl. 1985;14(2-3):171-6. doi: 10.3109/01485018508988294.
Washed human spermatozoa from 21 individuals with an average motility of 60% (quality index 277 +/- 16) had an endogenous ATP generation of 7.5 +/- 3.4 nmole/10(8) spermatozoa. The ATP concentrations in spermatozoa from 16 patients with severely impaired motility of 26% (quality index 98 +/- 13) was 16.9 +/- 9.9 nmole/10(8) spermatozoa. There was no correlation between ATP content and motility in either group. Sperm penetration into blood serum type AB, Rh-positive, was evaluated using a capillary tube penetration test. The penetration was graded with a maximum score of 14. Spermatozoa with an initial motility of 60% reached a score of 10 +/- 0.7. After addition of 20 mmole of ATP the score was significantly improved to 13 +/- 0.3. Compared with these results spermatozoa with an average motility of 26% reached a score of 4 +/- 1. Exogenous ATP (20 mmole) increased the score to 8 +/- 1.0. In both groups reduced glutathione had a negative effect. Human spermatozoa with high and low motility are capable to synthesize ATP. A dysfunction of the phosphorylating particles in the mitochondria appears not to be associated with low sperm motility.
来自21名个体的洗涤后人类精子，平均活力为60%（质量指数277±16），其内源性ATP生成量为7.5±3.4纳摩尔/10⁸个精子。16名严重活力受损（26%）（质量指数98±13）患者的精子中ATP浓度为16.9±9.9纳摩尔/10⁸个精子。两组中ATP含量与活力均无相关性。使用毛细管穿透试验评估精子对AB型、Rh阳性血清的穿透情况。穿透情况评分最高为14分。初始活力为60%的精子得分10±0.7。添加20毫摩尔ATP后，得分显著提高至13±0.3。与这些结果相比，平均活力为26%的精子得分4±1。外源性ATP（20毫摩尔）使得分提高到8±1.0。在两组中，还原型谷胱甘肽均有负面影响。高活力和低活力的人类精子都能够合成ATP。线粒体中磷酸化颗粒的功能障碍似乎与精子低活力无关。
