Adam Saber Y, Muniyappan Madesh, Huang Hao, Ennab Wael, Liu Hao-Yu, Ahmed Abdelkareem A, Sun Ming-An, Dessie Tadelle, Kim In Ho, Hu Yun, Luo Xugang, Cai Demin
Laboratory of Animal Physiology and Molecular Nutrition, Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou 225009, China.
Antioxidants (Basel). 2024 Sep 4;13(9):1079. doi: 10.3390/antiox13091079.
Heat stress (HS) is a significant concern in broiler chickens, which is vital for global meat supply in the dynamic field of poultry farming. The impact of heat stress on the ileum and its influence on the redox homeostatic genes in chickens remains unclear. We hypothesized that adding zinc to the feed of heat-stressed broilers would improve their resilience to heat stress. However, this study aimed to explore the effects of organic zinc supplementation under HS conditions on broiler chickens' intestinal histology and regulation of HS index genes. In this study, 512 Xueshan chickens were divided into four groups: vehicle, HS, 60 mg/kg zinc, and HS + 60 mg/kg zinc groups. Findings revealed that zinc supply positively increased the VH and VH: CD in the ileum of the broilers compared to the HS group, while CD and VW decreased in Zn and HS+Zn supplemented broilers. Zn administration significantly increased superoxide dismutase (), catalase (), glutathione (), and decreased the enzymatic activities of reactive oxygen species () and malondialdehyde (MDA) compared to the HS group. In addition, Zn administration significantly increased relative ATP, complex I, III, and V enzyme activity compared to the HS group. Furthermore, the expression of acyl-CoA synthetase long-chain family member 4 (), lactate transporter 3 (), peroxiredoxin (), and transferrin receptor () in the protein levels was extremely downregulated in HS+Zn compared to the HS group. Zn supply significantly decreased the enrichment of , , and at target loci of , , and compared to the HS group. The occupancies of histone active marks , , , and at the locus of and were significantly decreased in HS+Zn compared to the HS group. Moreover, and at the locus of and were significantly decreased in HS+Zn compared to the HS group. This study emphasizes that organic Zn is a potential strategy for modulating the oxidative genes , , , and in the ileum of chickens via nuclear receptor regulation and histone modifications.
热应激（HS）是肉鸡养殖中的一个重大问题，在充满活力的家禽养殖领域，肉鸡养殖对全球肉类供应至关重要。热应激对鸡回肠的影响及其对氧化还原稳态基因的影响仍不清楚。我们假设在热应激肉鸡的饲料中添加锌会提高它们对热应激的恢复力。然而，本研究旨在探讨在热应激条件下补充有机锌对肉鸡肠道组织学和热应激指数基因调控的影响。在本研究中，512只雪山鸡被分为四组：对照组、热应激组、60mg/kg锌组和热应激+60mg/kg锌组。研究结果显示，与热应激组相比，锌供应使肉鸡回肠中的绒毛高度（VH）和绒毛高度与隐窝深度比值（VH:CD）呈正向增加，而在补充锌和热应激+锌的肉鸡中，隐窝深度（CD）和绒毛宽度（VW）降低。与热应激组相比，补锌显著增加了超氧化物歧化酶（）、过氧化氢酶（）、谷胱甘肽（），并降低了活性氧（）和丙二醛（MDA）的酶活性。此外，与热应激组相比，补锌显著增加了相对ATP、复合体I、III和V的酶活性。此外，与热应激组相比，热应激+锌组中酰基辅酶A合成酶长链家族成员4（）、乳酸转运蛋白3（）、过氧化物酶（）和转铁蛋白受体（）的蛋白水平表达极度下调。与热应激组相比，锌供应显著降低了在、和靶位点处、和的富集。与热应激组相比，热应激+锌组在和位点处组蛋白活性标记、、、和的占有率显著降低。此外，与热应激组相比，热应激+锌组在和位点处的和显著降低。本研究强调，有机锌是通过核受体调控和组蛋白修饰来调节鸡回肠中氧化基因、、、和的一种潜在策略。
