准直和聚焦低强度脉冲超声刺激对兔下颌骨修复的影响。
Effects of collimated and focused low-intensity pulsed ultrasound stimulation on the mandible repair in rabbits.
作者信息
Liu Xiaohan, Hu Ying, Wu Lin, Li Shujun
机构信息
Department of Prosthodontics, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang 110002, China.
Department of Pediatric Dentistry, Dalian Stomatological Hospital, Dalian 116021, China.
出版信息
Ann Transl Med. 2020 Feb;8(4):98. doi: 10.21037/atm.2019.12.89.
BACKGROUND
This study was to evaluate the effects of low-intensity collimated pulse ultrasound (LICU) and low-intensity focused-pulse ultrasound (LIFU) stimulation on the osteogenesis in the porous silicon carbide (SiC) scaffold implanted in the rabbit mandible.
METHODS
Rabbits were randomly divided into LIFU group, LICU group and control group (without ultrasound treatment). The intensities of LICU and LIFU were 30 and 300 mW/cm, respectively. The subcutaneous and subperiosteal temperatures were measured continuously during the 20-min ultrasound treatment. Then, the porous SiC scaffolds were implanted into the mandible, followed by LICU or LIFU once daily, and the quantity and structure of bone were assessed by methylene blue-acid fuchsin staining and micro-CT at 3, 6 and 9 weeks after implantation.
RESULTS
The changes in the subcutaneous and subperiosteal temperatures during LICU and LIFU were less than 1 °C. The bone mass increased and the structure of bone tissues became more mature over time. The bone mass and mean pore occupancy fraction (POF) in the LIFU group were significantly greater than in the LICU group at three time points (P<0.05). Bone ingrowth in different directions was observed, and the new bone formation in the mesial, distal, top, and lingual sides of the implants in the LIFU group was greater than in the LICU group and control group (P<0.05).
CONCLUSIONS
LIFU and LICU may effectively promote bone formation in the mandible scaffold, and LIFU significantly accelerates bone formation in both buccal side and lingual side of the scaffold.
背景
本研究旨在评估低强度准直脉冲超声（LICU）和低强度聚焦脉冲超声（LIFU）刺激对植入兔下颌骨的多孔碳化硅（SiC）支架中成骨作用的影响。
方法
将兔子随机分为LIFU组、LICU组和对照组（未进行超声治疗）。LICU和LIFU的强度分别为30和300 mW/cm²。在20分钟的超声治疗过程中持续测量皮下和骨膜下温度。然后，将多孔SiC支架植入下颌骨，随后每天进行一次LICU或LIFU治疗，并在植入后3、6和9周通过亚甲基蓝-酸性品红染色和微型计算机断层扫描（micro-CT）评估骨的数量和结构。
结果
LICU和LIFU治疗期间皮下和骨膜下温度变化小于1℃。随着时间的推移，骨量增加，骨组织结构变得更加成熟。在三个时间点，LIFU组的骨量和平均孔隙占有率（POF）均显著高于LICU组（P<0.05）。观察到不同方向的骨长入，LIFU组植入物近中、远中、顶部和舌侧的新骨形成均大于LICU组和对照组（P<0.05）。
结论
LIFU和LICU均可有效促进下颌骨支架中的骨形成，且LIFU可显著加速支架颊侧和舌侧的骨形成。
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