• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

诱导毒素表达下的持续性与可培养性

Persistence and Culturability of under Induced Toxin Expression.

作者信息

Dhaouadi Yousr, Hashemi Mohamad Javad, Ren Dacheng

机构信息

Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY 13244, USA.

BioInspired Institute, Syracuse University, Syracuse, NY 13244, USA.

出版信息

Antibiotics (Basel). 2024 Sep 9;13(9):863. doi: 10.3390/antibiotics13090863.

DOI:10.3390/antibiotics13090863
PMID:39335036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11428644/
Abstract

BACKGROUND/OBJECTIVES: Bacteria are well known to enter dormancy under stress conditions. However, the mechanisms of different dormancy-related phenotypes are still under debate and many questions remain unanswered. This study aims to better understand the effects of toxin gene expression on the dormancy of .

METHODS

The effects of toxin gene expression on growth, persistence, and culturability were characterized. Specifically, we detailed dose- and time-dependent dormancy of and its susceptibility to ofloxacin via arabinose-induced toxin gene expression under the promoter. A new plot was developed to better describe the dynamic changes in culturability and persistence. The expression level of was determined using qPCR and cellular activities were monitored using fluorescence imaging and flow cytometry.

RESULTS

High-level persister formation and strong tolerance to ofloxacin were observed after high-level induction. The new plot reveals more information than the changes in persistence alone, e.g., reduced culturability of and thus deeper dormancy under high-level induction. Consistently, controlled induction led to decreased cellular activities at promoter and an increase in the non-culturable subpopulation.

CONCLUSIONS

Overall, this study provides new insights into dormancy induced by toxin gene expression and a more comprehensive view of persistence and culturability. The findings may help develop better control agents against dormant bacterial cells.

摘要

背景/目的：众所周知，细菌在应激条件下会进入休眠状态。然而，不同休眠相关表型的机制仍存在争议，许多问题仍未得到解答。本研究旨在更好地了解毒素基因表达对[具体细菌名称未给出]休眠的影响。

方法

对毒素基因表达对生长、持续存活和可培养性的影响进行了表征。具体而言，我们详细研究了在[具体启动子名称未给出]启动子下，通过阿拉伯糖诱导[具体毒素基因名称未给出]毒素基因表达，[具体细菌名称未给出]的剂量和时间依赖性休眠及其对氧氟沙星的敏感性。开发了一种新的图表来更好地描述可培养性和持续存活的动态变化。使用qPCR测定[具体基因名称未给出]的表达水平，并使用荧光成像和流式细胞术监测细胞活性。

结果

在高水平[具体毒素名称未给出]诱导后，观察到高水平持留菌的形成和对氧氟沙星的强耐受性。新图表揭示的信息比仅持续存活的变化更多，例如，在高水平[具体毒素名称未给出]诱导下，[具体细菌名称未给出]的可培养性降低，从而休眠更深。一致地，受控的[具体毒素名称未给出]诱导导致在[具体启动子名称未给出]启动子处细胞活性降低，以及不可培养亚群增加。

结论

总体而言，本研究为毒素基因表达诱导的休眠提供了新的见解，并对持续存活和可培养性有了更全面的认识。这些发现可能有助于开发更好的针对休眠细菌细胞的控制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/0bfb2825de00/antibiotics-13-00863-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/06c6df680344/antibiotics-13-00863-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/c9788e382d1e/antibiotics-13-00863-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/2b4d3f9da603/antibiotics-13-00863-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/bba036ba9dc3/antibiotics-13-00863-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/38d164df59c8/antibiotics-13-00863-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/d1a5f20ed4aa/antibiotics-13-00863-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/b0580d02c1fb/antibiotics-13-00863-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/0bfb2825de00/antibiotics-13-00863-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/06c6df680344/antibiotics-13-00863-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/c9788e382d1e/antibiotics-13-00863-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/2b4d3f9da603/antibiotics-13-00863-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/bba036ba9dc3/antibiotics-13-00863-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/38d164df59c8/antibiotics-13-00863-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/d1a5f20ed4aa/antibiotics-13-00863-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/b0580d02c1fb/antibiotics-13-00863-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71f4/11428644/0bfb2825de00/antibiotics-13-00863-g008.jpg

相似文献

1
Persistence and Culturability of under Induced Toxin Expression.诱导毒素表达下的持续性与可培养性
Antibiotics (Basel). 2024 Sep 9;13(9):863. doi: 10.3390/antibiotics13090863.
2
Ectopic overexpression of wild-type and mutant hipA genes in Escherichia coli: effects on macromolecular synthesis and persister formation.野生型和突变型hipA基因在大肠杆菌中的异位过表达：对大分子合成和持留菌形成的影响。
J Bacteriol. 2006 Jun;188(11):3826-36. doi: 10.1128/JB.01740-05.
3
Phylogeny Reveals Novel HipA-Homologous Kinase Families and Toxin-Antitoxin Gene Organizations.系统发育揭示了新型 HipA 同源激酶家族和毒素-抗毒素基因组织。
mBio. 2021 Jun 29;12(3):e0105821. doi: 10.1128/mBio.01058-21. Epub 2021 Jun 1.
4
Reassessing the Role of Type II Toxin-Antitoxin Systems in Formation of Escherichia coli Type II Persister Cells.重新评估 II 型毒素-抗毒素系统在大肠埃希菌 II 型持留细胞形成中的作用。
mBio. 2018 Jun 12;9(3):e00640-18. doi: 10.1128/mBio.00640-18.
5
Serine-Threonine Kinases Encoded by Split Homologs Inhibit Tryptophanyl-tRNA Synthetase.裂位同源编码的丝氨酸-苏氨酸激酶抑制色氨酰-tRNA 合成酶。
mBio. 2019 Jun 18;10(3):e01138-19. doi: 10.1128/mBio.01138-19.
6
Kinase activity of overexpressed HipA is required for growth arrest and multidrug tolerance in Escherichia coli.大肠杆菌中生长停滞和多药耐受性需要过表达的HipA的激酶活性。
J Bacteriol. 2006 Dec;188(24):8360-7. doi: 10.1128/JB.01237-06. Epub 2006 Oct 13.
7
The Dynamic Transition of Persistence toward the Viable but Nonculturable State during Stationary Phase Is Driven by Protein Aggregation.在稳定期，持久性向可生存但不可培养状态的动态转变是由蛋白质聚集驱动的。
mBio. 2021 Aug 31;12(4):e0070321. doi: 10.1128/mBio.00703-21. Epub 2021 Aug 3.
8
Novel Inhibitors of Toxin HipA Reduce Multidrug Tolerant Persisters.毒素HipA的新型抑制剂可减少多药耐受持久性细菌。
ACS Med Chem Lett. 2016 Mar 13;7(5):449-53. doi: 10.1021/acsmedchemlett.5b00420. eCollection 2016 May 12.
9
Stochastic induction of persister cells by HipA through (p)ppGpp-mediated activation of mRNA endonucleases.HipA通过（p）ppGpp介导的mRNA核酸内切酶激活对持留菌细胞进行随机诱导。
Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):5171-6. doi: 10.1073/pnas.1423536112. Epub 2015 Apr 6.
10
Quantitative analysis of persister fractions suggests different mechanisms of formation among environmental isolates of E. coli.定量分析持久细胞分数表明大肠杆菌环境分离株形成的不同机制。
BMC Microbiol. 2013 Feb 4;13:25. doi: 10.1186/1471-2180-13-25.

引用本文的文献

1
Type II Toxin-Antitoxin Systems in .中的II型毒素-抗毒素系统
Infect Drug Resist. 2025 Feb 24;18:1083-1096. doi: 10.2147/IDR.S501485. eCollection 2025.

本文引用的文献

1
Bacterial persisters: molecular mechanisms and therapeutic development.细菌持久态：分子机制与治疗开发。
Signal Transduct Target Ther. 2024 Jul 17;9(1):174. doi: 10.1038/s41392-024-01866-5.
2
Antimicrobial Resistance: A Growing Serious Threat for Global Public Health.抗菌药物耐药性：对全球公共卫生日益严重的威胁。
Healthcare (Basel). 2023 Jul 5;11(13):1946. doi: 10.3390/healthcare11131946.
3
Combatting persister cells: The daunting task in post-antibiotics era.对抗持留菌：抗生素后时代的艰巨任务。
Cell Insight. 2023 Apr 24;2(4):100104. doi: 10.1016/j.cellin.2023.100104. eCollection 2023 Aug.
4
Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy.存活但不可培养（VBNC）状态，一种被低估和有争议的微生物生存策略。
Trends Microbiol. 2023 Oct;31(10):1013-1023. doi: 10.1016/j.tim.2023.04.009. Epub 2023 May 23.
5
Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms.活的但不可培养的细菌及其复苏：对培养未培养海洋微生物的意义。
Mar Life Sci Technol. 2020 May 13;3(2):189-203. doi: 10.1007/s42995-020-00041-3. eCollection 2021 May.
6
Immune Response Modulation by Pseudomonas aeruginosa Persister Cells.铜绿假单胞菌持续生存细胞对免疫反应的调节。
mBio. 2023 Apr 25;14(2):e0005623. doi: 10.1128/mbio.00056-23. Epub 2023 Mar 15.
7
Macrophage internalization creates a multidrug-tolerant fungal persister reservoir and facilitates the emergence of drug resistance.巨噬细胞内化会产生一个多药耐受真菌持久存贮库，并促进耐药性的出现。
Nat Commun. 2023 Mar 2;14(1):1183. doi: 10.1038/s41467-023-36882-6.
8
Wake Up! Resuscitation of Viable but Nonculturable Bacteria: Mechanism and Potential Application.苏醒！可存活但不可培养细菌的复苏：机制与潜在应用
Foods. 2022 Dec 23;12(1):82. doi: 10.3390/foods12010082.
9
Integrative biology of persister cell formation: molecular circuitry, phenotypic diversification and fitness effects.持留细胞形成的综合生物学：分子电路、表型多样化和适应度效应。
J R Soc Interface. 2022 Sep;19(194):20220129. doi: 10.1098/rsif.2022.0129. Epub 2022 Sep 14.
10
Metabolic characteristics and markers in viable but nonculturable state of Pseudomonas aeruginosa induced by chlorine stress.氯胁迫诱导铜绿假单胞菌活但不可培养状态的代谢特征和标志物。
Environ Res. 2022 Nov;214(Pt 3):114111. doi: 10.1016/j.envres.2022.114111. Epub 2022 Aug 17.