Mencía-Ares Oscar, Ramos-Calvo Eva, González-Fernández Alba, Aguarón-Turrientes Álvaro, Pastor-Calonge Ana Isabel, Miguélez-Pérez Rubén, Gutiérrez-Martín César B, Martínez-Martínez Sonia
Department of Animal Health, Faculty of Veterinary, Universidad de León, 24007 León, Spain.
Laboratorios SYVA, 24009 León, Spain.
Antibiotics (Basel). 2024 Sep 11;13(9):871. doi: 10.3390/antibiotics13090871.
is a significant pathogen in swine, primarily causing exudative epidermitis. Addressing infections requires both the characterization of virulence and antimicrobial resistance (AMR) in farm-recovered isolates. This study aimed to characterize the virulence, AMR, and biofilm formation of isolates from Spanish swine farms. A total of 49 isolates were analyzed, originating from animals with cutaneous, reproductive, and systemic clinical signs. Half of the isolates (49.0%) were positive for at least one virulence factor (VF) gene, with being the most frequent (28.6%). A high frequency of multidrug resistant (MDR) isolates was observed (83.7%), with significant resistance to commonly used antimicrobials, including lincosamides (83.7%), pleuromutilins (81.6%), penicillins (75.5%), and tetracyclines (73.5%). All isolates exhibited robust in vitro biofilm formation capacity (DC = 15.6 ± 7.0). Significant associations were found between VFs, biofilm formation, and AMR patterns, highlighting the link between the resistance to lincosamides and pleuromutilins ( < 0.001; Φ = 0.57) and macrolides ( < 0.001; Φ = 0.48), and the association of AMR with the and VF genes. These findings underscore the need for targeted diagnostics to improve management and therapeutic strategies to mitigate the impact of on swine production.
是猪的一种重要病原体，主要引起渗出性皮炎。应对感染需要对农场分离株的毒力和抗菌药物耐药性（AMR）进行表征。本研究旨在表征西班牙猪场分离株的毒力、AMR和生物膜形成情况。共分析了49株分离株，它们来自有皮肤、生殖和全身临床症状的动物。一半的分离株（49.0%）至少对一种毒力因子（VF）基因呈阳性，其中最为常见（28.6%）。观察到多药耐药（MDR）分离株的频率很高（83.7%），对常用抗菌药物有显著耐药性，包括林可酰胺类（83.7%）、截短侧耳素类（81.6%）、青霉素类（75.5%）和四环素类（73.5%）。所有分离株在体外均表现出强大的生物膜形成能力（DC = 15.6 ± 7.0）。在VF、生物膜形成和AMR模式之间发现了显著关联，突出了对林可酰胺类和截短侧耳素类耐药性之间的联系（< 0.001；Φ = 0.57）以及与大环内酯类耐药性之间的联系（< 0.001；Φ = 0.48），以及AMR与 和 VF基因之间的关联。这些发现强调了需要有针对性的诊断方法来改进管理和治疗策略，以减轻其对猪生产的影响。
