Lee Seong Shin, Wi Jisoo, Kim Hyun Sang, Seong Pil Nam, Lee Sung Dae, Kim Jungeun, Lee Yookyung
Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55368, Republic of Korea.
Animal Products and Processing Division, National Institute of Animal Science, Wanju 55368, Republic of Korea.
Animals (Basel). 2024 Sep 11;14(18):2637. doi: 10.3390/ani14182637.
This study investigated the impact of root (RP) for reducing methane and its impact on rumen fermentation and blood metabolites in cattle. Rumen fluid was collected from three cannulated steers (736 ± 15 kg) and mixed with buffer (1:3 ratio) for the in vitro trial. Treatments were divided into control and RP supplement groups (1%, 3%, and 5% of substrates), with each sample incubated at 39 °C for 24 and 48 hours. Methane was measured after incubation, showing a dose-dependent linear decrease after 48 hours. Quadratic changes were observed in total volatile fatty acids, acetate, and butyrate. Additionally, in vitro dry matter digestibility decreased linearly with RP inclusion. In vivo trials involved four Korean steers in a 2 × 2 crossover design over 3 weeks, with treatments including a control group and a group with 3% RP addition. Dry matter intake (DMI) tended to decrease in the RP group compared to the control. Methane emissions (g/kg DMI) were not affected by RP addition. Blood metabolites indicated higher lipase concentrations in the RP group. In conclusion, RP reduced methane production in the in vitro trial but had no effect in the in vivo trial, likely due to adaptation of ruminal bacteria to RP.
本研究调查了根粉（RP）对减少甲烷的影响及其对牛瘤胃发酵和血液代谢物的影响。从三头安装了瘤胃瘘管的阉牛（736±15千克）采集瘤胃液，并与缓冲液按1:3的比例混合用于体外试验。处理分为对照组和RP添加组（底物的1%、3%和5%），每个样品在39℃下孵育24小时和48小时。孵育后测量甲烷，结果显示48小时后呈剂量依赖性线性下降。总挥发性脂肪酸、乙酸和丁酸出现二次变化。此外，体外干物质消化率随RP添加量呈线性下降。体内试验采用2×2交叉设计，对四头韩国阉牛进行为期3周的试验，处理包括对照组和添加3%RP的组。与对照组相比，RP组的干物质摄入量（DMI）有下降趋势。添加RP对甲烷排放量（克/千克DMI）没有影响。血液代谢物表明RP组的脂肪酶浓度较高。总之，在体外试验中RP减少了甲烷产生，但在体内试验中没有效果，这可能是由于瘤胃细菌对RP产生了适应性。
