Poonlaphdecha Srisupaph, Ribas Alexis, Chaisiri Kittipong, Morand Serge, Chan Abigail Hui En, Thaenkham Urusa
Parasitology Section, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Science, Institut de Recerca de la Biodiversitat (IRBio), University of Barcelona, 08028 Barcelona, Spain.
Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Animals (Basel). 2024 Sep 14;14(18):2674. doi: 10.3390/ani14182674.
, a parasitic worm infecting rodents, is globally prevalent in black rats, brown rats, and house mice. It is hypothesized to originate from Asia due to its widespread presence in Southeast Asia in various Murinae. Previous molecular studies focused on European, African, and Japanese specimens, but none included samples from the putative native range. Rodents were collected between 2008 and 2015 across various localities in Southeast Asia and Europe, identified by morphology or genetic barcoding. Viscera were examined or preserved for later inspection. DNA was extracted from . PCR amplification targeting the mtCOI gene and ITS1 region was conducted in this study using newly designed primers (based on reference sequences). PCR amplicons were subsequently sequenced and analyzed. In this study, the phylogenetic analysis using ITS1 sequences revealed that samples from Thai and Laotian rodents belong to the species , exhibiting low genetic variation compared to samples from other regions. Genetic distance calculations using mtCOI sequences confirmed the marked distinction of from other species. Our phylogenetic analyses using partial mtCOI and ITS1 sequences have significantly enhanced our comprehension of the genetic diversity and evolutionary history of the nematode .
一种感染啮齿动物的寄生虫，在黑鼠、褐鼠和家鼠中全球普遍存在。由于其在东南亚各种鼠科动物中的广泛存在，据推测它起源于亚洲。先前的分子研究集中在欧洲、非洲和日本的标本上，但没有包括来自假定原生范围的样本。在2008年至2015年期间，在东南亚和欧洲的不同地点收集啮齿动物，通过形态学或基因条形码进行鉴定。检查或保存内脏以供后续检查。从……中提取DNA。本研究使用新设计的引物（基于……参考序列）对线粒体细胞色素氧化酶亚基I（mtCOI）基因和内转录间隔区1（ITS1）区域进行PCR扩增。随后对PCR扩增产物进行测序和分析。在本研究中，使用ITS1序列进行的系统发育分析表明，来自泰国和老挝啮齿动物的……样本属于……物种，与来自其他地区的样本相比，遗传变异较低。使用mtCOI序列进行的遗传距离计算证实了……与其他……物种的明显区别。我们使用部分mtCOI和ITS1序列进行的系统发育分析显著提高了我们对线虫……的遗传多样性和进化历史的理解。
