Le Faouder Julie, Guého Aurélie, Lavigne Régis, Wauquier Fabien, Boutin-Wittrant Line, Bouvret Elodie, Com Emmanuelle, Wittrant Yohann, Pineau Charles
Abyss Ingredients, 860 Route de Caudan, 56850 Caudan, France.
Univ Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, Protim core facility, F-35000 Rennes, France.
Biomedicines. 2024 Sep 19;12(9):2132. doi: 10.3390/biomedicines12092132.
: Marine collagen peptides (MCPs) and glycosaminoglycans (GAGs) have been described as potential wound-healing (WH) agents. Fish cartilage hydrolysate (FCH) is a natural active food ingredient obtained from enzymatic hydrolysis which combines MCPs and GAGs. Recently, the clinical benefits of FCH supplementation for the skin, as well as its mode of action, have been demonstrated. Some of the highlighted mechanisms are common to the WH process. The aim of the study is therefore to investigate the influence of FCH supplementation on the skin healing processes and the underlying mechanisms. : To this end, an ex vivo clinical approach, which takes into account the clinical digestive course of nutrients, coupled with primary cell culture on human dermal fibroblasts (HDFs) and ultra-deep proteomic analysis, was performed. The effects of human serum enriched in circulating metabolites resulting from FCH ingestion (FCH-enriched serum) were assessed on HDF WH via an in vitro scratch wound assay and on the HDF proteome via diaPASEF (Data Independent Acquisition-Parallel Accumulation Serial Fragmentation) proteomic analysis. : Results showed that FCH-enriched human serum accelerated wound closure. In support, proteins with anti-inflammatory and immunomodulatory properties and proteins prone to promote hydration and ECM stability showed increased expression in HDFs after exposure to FCH-enriched serum. : Taken together, these data provide valuable new insights into the mechanisms that may contribute to FCH's beneficial impact on human skin functionality by supporting WH. Further studies are needed to reinforce these preliminary data and investigate the anti-inflammatory and immunomodulatory properties of FCH.
海洋胶原蛋白肽（MCPs）和糖胺聚糖（GAGs）已被描述为潜在的伤口愈合（WH）剂。鱼软骨水解物（FCH）是一种通过酶水解获得的天然活性食品成分，它结合了MCPs和GAGs。最近，补充FCH对皮肤的临床益处及其作用方式已得到证实。一些突出的机制与伤口愈合过程相同。因此，本研究的目的是探讨补充FCH对皮肤愈合过程及其潜在机制的影响。为此，采用了一种体外临床方法，该方法考虑了营养物质的临床消化过程，并结合了人皮肤成纤维细胞（HDFs）的原代细胞培养和超深度蛋白质组学分析。通过体外划痕试验评估摄入FCH后富含循环代谢物的人血清（富含FCH的血清）对HDF伤口愈合的影响，并通过diaPASEF（数据独立采集-平行累积串联碎片化）蛋白质组学分析评估对HDF蛋白质组的影响。结果表明，富含FCH的人血清加速了伤口闭合。此外，具有抗炎和免疫调节特性的蛋白质以及易于促进水合作用和细胞外基质稳定性的蛋白质在暴露于富含FCH的血清后在HDFs中的表达增加。综上所述，这些数据为FCH通过支持伤口愈合对人体皮肤功能产生有益影响的机制提供了有价值的新见解。需要进一步的研究来加强这些初步数据，并研究FCH的抗炎和免疫调节特性。
