Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan.
Department of Nursing, Division of Basic Medical Sciences, Chang Gung University of Science and Technology, Chiayi 61363, Taiwan.
Mar Drugs. 2020 Oct 11;18(10):511. doi: 10.3390/md18100511.
Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of and its effect on in vitro and in vivo wound healing was compared to type I collagen derived from tilapia skin. Sturgeon cartilage collagen (SCC) was composed of α1 chains and with a thermal denaturation (T) at 22.5 and melting temperature (T) at 72.5 °C. Coating SCC potentiated proliferation, migration, and invasion of human dermal fibroblast adult (HDFa) cells. Furthermore, SCC upregulated the gene expression of extracellular matrix (ECM) components (col Iα1, col IIIα1, elastin, and Has2) and epithelial-mesenchymal transition (EMT) molecules (N-cadherin, Snail, and MMP-1) in HDFa. Pretreatment with Akt and mitogen-activated protein kinase (MAPK) inhibitors significantly attenuated the HDFa invasion caused by SCC. In mice, the application of SCC on dorsal wounds effectively facilitated wound healing as evidenced by 40-59% wound contraction, whereas the untreated wounds were 18%. We observed that SCC reduced inflammation, promoted granulation, tissue formation, and ECM deposition, as well as re-epithelialization in skin wounds. In addition, SCC markedly upregulated the production of growth factors in the dermis, and dermal and subcutaneous white adipose tissue; in contrast, the administration of tilapia skin collagen (TSC) characterized by typical type I collagen was mainly expressed in the epidermis. Collectively, these findings indicate SCC accelerated wound healing by targeting fibroblast in vitro and in vivo.
型胶原蛋白是软骨的重要组成部分；然而，其对皮肤伤口愈合的影响知之甚少。在这项研究中，从软骨中提取了型胶原蛋白，并将其与从罗非鱼皮中提取的型胶原蛋白进行了比较，以研究其对体外和体内伤口愈合的影响。鲟鱼软骨胶原蛋白（SCC）由α1 链组成，热变性（T）为 22.5，熔化温度（T）为 72.5°C。SCC 涂层可促进人真皮成纤维细胞（HDFa）的增殖、迁移和侵袭。此外，SCC 上调了细胞外基质（ECM）成分（col Iα1、col IIIα1、弹性蛋白和 Has2）和上皮-间充质转化（EMT）分子（N-钙粘蛋白、Snail 和 MMP-1）在 HDFa 中的表达。用 Akt 和丝裂原活化蛋白激酶（MAPK）抑制剂预处理可显著减弱 SCC 引起的 HDFa 侵袭。在小鼠中，SCC 应用于背部伤口可有效促进伤口愈合，伤口收缩率为 40-59%，而未处理的伤口为 18%。我们观察到 SCC 减少了炎症、促进了肉芽组织形成、组织形成和 ECM 沉积，以及皮肤伤口的再上皮化。此外，SCC 还显著上调了真皮、真皮和皮下白色脂肪组织中生长因子的产生；相比之下，主要表达在表皮的罗非鱼皮胶原蛋白（TSC）给药。总之，这些发现表明 SCC 通过靶向成纤维细胞在体外和体内加速了伤口愈合。
