Wang Shumin, Wang Pengjie, Liu Siyuan, Wang Ran, Li Yixuan, Wang Xiaoyu, Ren Fazheng, Luo Jie, Fang Bing
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
Department of Food and Bioengineering, Beijing Vocational College of Agriculture, Beijing 102442, China.
Foods. 2024 Sep 13;13(18):2900. doi: 10.3390/foods13182900.
In this work, a novel reinforcing filler, millet gliadin (MG), was used for the improvement of the mechanical properties of zein nanofibers. The structural and physicochemical properties of MG were compared with those of zein, and the influence of MG on the morphology, physical properties, and molecular structure of zein nanofibers was investigated. The results indicated that MG has an obviously smaller weight-average molecular weight (7623) in comparison to zein (13,330). Transmission electron microscopy showed that zein molecules more easily form aggregates with larger diameters than MG molecules in acetic acid. At a concentration of 30% (/), MG exhibited a significantly higher viscosity (0.66 ± 0.03 Pa·s) than zein (0.32 ± 0.01 Pa·s), indicating the stronger interactions of MG molecules. With the incorporation of MG, the tensile strength was significantly increased to 49.32 MPa (ZM-1/2), which is 2.08 times and 4.45 times higher than that of pure zein nanofibers (ZM-1/0) and MG nanofibers (ZM-0/1-1), respectively. Moreover, zein/MG composite nanofibers exhibited improved water stability. Fourier transform infrared spectra showed evidence of the hydrogen bonding interaction between zein and MG. Therefore, MG is a good candidate for use as a natural reinforcing filler in electrospun nanofibers made of biopolymers.
在本研究中，一种新型增强填料——小米醇溶蛋白（MG）被用于改善玉米醇溶蛋白纳米纤维的机械性能。将MG的结构和物理化学性质与玉米醇溶蛋白进行了比较，并研究了MG对玉米醇溶蛋白纳米纤维的形态、物理性质和分子结构的影响。结果表明，与玉米醇溶蛋白（13330）相比，MG的重均分子量明显更小（7623）。透射电子显微镜显示，在乙酸中，玉米醇溶蛋白分子比MG分子更容易形成直径更大的聚集体。在浓度为30%（/）时，MG的粘度（0.66±0.03 Pa·s）明显高于玉米醇溶蛋白（0.32±0.01 Pa·s），表明MG分子间的相互作用更强。随着MG的加入，拉伸强度显著提高到49.32 MPa（ZM-1/2），分别比纯玉米醇溶蛋白纳米纤维（ZM-1/0）和MG纳米纤维（ZM-0/1-1）高2.08倍和4.45倍。此外，玉米醇溶蛋白/MG复合纳米纤维表现出更好的水稳定性。傅里叶变换红外光谱显示了玉米醇溶蛋白和MG之间存在氢键相互作用的证据。因此，MG是用作由生物聚合物制成的电纺纳米纤维中的天然增强填料的良好候选材料。
