Wang Shumin, Li Jingyu, Wang Pengjie, Zhang Ming, Liu Siyuan, Wang Ran, Li Yixuan, Ren Fazheng, Fang Bing
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.
Foods. 2024 May 20;13(10):1583. doi: 10.3390/foods13101583.
Volatile active ingredients in biopolymer nanofibers are prone to burst and uncontrolled release. In this study, we used electrospinning and crosslinking to design a new sustained-release active packaging containing zein and eugenol (EU). Vapor-phase glutaraldehyde (GTA) was used as the crosslinker. Characterization of the crosslinked zein nanofibers was conducted via scanning electron microscopy (SEM), mechanical properties, water resistance, and Fourier transform infrared (FT-IR) spectroscopy. It was observed that crosslinked zein nanofibers did not lose their fiber shape, but the diameter of the fibers increased. By increasing the crosslink time, the mechanical properties and water resistance of the crosslinked zein nanofibers were greatly improved. The FT-IR results demonstrated the formation of chemical bonds between free amino groups in zein molecules and aldehyde groups in GTA molecules. EU was added to the zein nanofibers, and the corresponding release behavior in PBS was investigated using the dialysis membrane method. With an increase in crosslink time, the release rate of EU from crosslinked zein nanofibers decreased. This study demonstrates the potential of crosslinking by GTA vapors on the controlled release of the zein encapsulation structure containing EU. Such sustainable-release nanofibers have promising potential for the design of fortified foods or as active and smart food packaging.
生物聚合物纳米纤维中的挥发性活性成分易于突发和不受控制地释放。在本研究中，我们采用静电纺丝和交联技术设计了一种含有玉米醇溶蛋白和丁香酚（EU）的新型缓释活性包装。气相戊二醛（GTA）用作交联剂。通过扫描电子显微镜（SEM）、机械性能、耐水性和傅里叶变换红外（FT-IR）光谱对交联玉米醇溶蛋白纳米纤维进行了表征。观察到交联玉米醇溶蛋白纳米纤维没有失去其纤维形状，但纤维直径增加。通过增加交联时间，交联玉米醇溶蛋白纳米纤维的机械性能和耐水性得到了极大改善。FT-IR结果表明玉米醇溶蛋白分子中的游离氨基与GTA分子中的醛基之间形成了化学键。将EU添加到玉米醇溶蛋白纳米纤维中，并使用透析膜法研究了其在磷酸盐缓冲液（PBS）中的相应释放行为。随着交联时间的增加，EU从交联玉米醇溶蛋白纳米纤维中的释放速率降低。本研究证明了GTA蒸汽交联对含EU的玉米醇溶蛋白包封结构的控释潜力。这种缓释纳米纤维在强化食品设计或作为活性和智能食品包装方面具有广阔的应用前景。
