Kim Eun-A, Kang Nalae, Heo Jun-Ho, Park Areumi, Heo Seong-Yeong, Ko Chang-Ik, Ahn Yong-Seok, Ahn Ginnae, Heo Soo-Jin
Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of Korea.
Choung Ryong Fisheries Co., Ltd., Jeju 63612, Republic of Korea.
Foods. 2024 Sep 13;13(18):2902. doi: 10.3390/foods13182902.
Abalone, a marine edible gastropod with nutritional value, is a popular seafood delicacy worldwide, especially in Asia; however, viscera by-products are generally discarded during processing. Therefore, we investigated the skin health benefits of abalone viscera ultrasonic extract (AVU) in human dermal fibroblasts (HDFs) and human keratinocyte (HaCaT) cells. AVU showed valuable protein contents, indicating that it is a worthy and safe material for industrial application. AVU increased collagen synthesis production and messenger RNA (mRNA) expression of Collagen Type I Alpha 1, 2, and 3 chains through the transforming growth factor beta/suppressor of mother against the decapentaplegic pathway in HDF cells. AVU also increased hyaluronic acid production, upregulated Hyaluronan Synthases 1, 2, and 3, filaggrin and aquaporin3 mRNA levels, and downregulated hyaluronidase mRNA levels in HaCaT cells. Furthermore, mechanistic studies showed that AVU increased the phosphorylation of extracellular signal-regulated kinase, p38, and cyclic AMP response-binding protein activation. AVU activated the transcription factors, phosphoinositide 3-kinase, protein kinase B, and nuclear factor kappa B cell p65 and downregulated the degranulation of inhibitory kappa B in HaCaT cells. Studies of hyaluronic acid production in AVU by inhibiting EKR, p38 and NF-κB have shown that p38 MAPK and NF-κB signaling are pivotal mechanisms, particularly in the AVU. These results demonstrated that AVU produced from by-products may improve skin health and may thus be used as a functional food and cosmetics ingredient.
鲍鱼是一种具有营养价值的可食用海洋腹足纲动物，是全球尤其是亚洲广受欢迎的海鲜佳肴；然而，其内脏副产品在加工过程中通常被丢弃。因此，我们研究了鲍鱼内脏超声提取物（AVU）对人皮肤成纤维细胞（HDFs）和人角质形成细胞（HaCaT）的皮肤健康益处。AVU显示出有价值的蛋白质含量，表明它是一种值得且安全的工业应用材料。AVU通过转化生长因子β/抗五体不全母源抑制因子途径增加了HDF细胞中I型胶原蛋白α1、α2和α3链的胶原蛋白合成产量和信使核糖核酸（mRNA）表达。AVU还增加了HaCaT细胞中的透明质酸产量，上调了透明质酸合酶1、2和3、丝聚合蛋白和水通道蛋白3的mRNA水平，并下调了透明质酸酶的mRNA水平。此外，机制研究表明，AVU增加了细胞外信号调节激酶、p38和环磷酸腺苷反应结合蛋白的磷酸化。AVU激活了转录因子、磷脂酰肌醇3激酶、蛋白激酶B和核因子κB细胞p65，并下调了HaCaT细胞中抑制性κB的脱颗粒。通过抑制EKR、p38和NF-κB对AVU中透明质酸产生的研究表明，p38丝裂原活化蛋白激酶和NF-κB信号传导是关键机制，尤其是在AVU中。这些结果表明，由副产品产生的AVU可能改善皮肤健康，因此可用作功能性食品和化妆品成分。
