Gordon S G, Hasiba U, Cross B A, Poole M A, Falanga A
Blood. 1985 Dec;66(6):1261-5.
Various coagulation abnormalities are associated with pregnancy. Several investigators have suggested that there may be a unique procoagulant associated with amniotic tissue and fluid. We identified a cysteine proteinase from malignant tissue, cancer procoagulant, and had reason to believe a similar proteinase may be present in amniotic tissue. Amniotic fluid and extracts of amnion-chorion were purified by immunoaffinity chromatography with an antibody that was developed to cancer procoagulant antigen. The purified amnion-chorion procoagulant initiated clotting in normal and factor VII-deficient citrated human plasma and directly activated pure human factor X in a two-stage clotting assay. It was inhibited by 1 mmol/L of iodoacetamide and 0.1 mmol/L of HgCl2, and the procoagulant activity was activated by 10 mmol/L of KCN; these are classic properties of cysteine proteinases. The pure amnion-chorion procoagulant had the same mol wt and immunologic determinants as cancer procoagulant from rabbit V2 carcinoma, as determined by crossed immunodiffusion, suggesting that the same or very similar proteins were associated with both tissues. Thus, this procoagulant may be derived from both undifferentiated and dedifferentiated cells.
多种凝血异常与妊娠相关。一些研究者认为，可能存在一种与羊膜组织和羊水相关的独特促凝剂。我们从恶性组织中鉴定出一种半胱氨酸蛋白酶——癌促凝剂，并有理由相信羊膜组织中可能存在类似的蛋白酶。用针对癌促凝剂抗原制备的抗体，通过免疫亲和层析法对羊水和羊膜 - 绒毛膜提取物进行纯化。纯化后的羊膜 - 绒毛膜促凝剂可在正常和缺乏因子VII的枸橼酸化人血浆中引发凝血，并在两阶段凝血试验中直接激活纯人因子X。它受到1 mmol/L碘乙酰胺和0.1 mmol/L HgCl2的抑制，其促凝活性被10 mmol/L KCN激活；这些是半胱氨酸蛋白酶的典型特性。通过交叉免疫扩散测定，纯化后的羊膜 - 绒毛膜促凝剂与兔V2癌的癌促凝剂具有相同的分子量和免疫决定簇，这表明两种组织中存在相同或非常相似的蛋白质。因此，这种促凝剂可能来源于未分化和去分化细胞。
