Feng Feng, Li Qilong, Sun Xuefei, Yao Li, Wang Xiuyu
Institute of Process Equipment, College of Energy Engineering, Zhejiang University, Hangzhou 310027, China.
Institute of Chemistry Chinese Academy of Sciences, Beijing 100190, China.
Biology (Basel). 2024 Aug 25;13(9):658. doi: 10.3390/biology13090658.
Cancer cells display elevated reactive oxygen species (ROS) and altered redox status. Herein, based on these characteristics, we present a multi-drug delivery platform, AMB@PDAP-Fe (APPF), from the magnetotactic bacterium AMB-1 and realize MRI-visualized tumor-microenvironment-responsive photothermal-chemodynamic therapy. The Fe in PDAP-Fe is reduced by the GSH at the tumor site and is released in the form of highly active Fe, which catalyzes the generation of ROS through the Fenton reaction and inhibits tumor growth. At the same time, the significant absorption of the mineralized magnetosomes in AMB-1 cells in the NIR region enables them to efficiently convert near-infrared light into heat energy for photothermal therapy (PTT), to which PDAP also contributes. The heat generated in the PTT process accelerates the process of Fe release, thereby achieving an enhanced Fenton reaction in the tumor microenvironment. In addition, the magnetosomes in AMB-1 are used as an MRI contrast agent, and the curing process is visualized. This tumor microenvironment-responsive MTB-based multi-drug delivery platform displays the potency to combat tumors and demonstrates the utility and practicality of understanding the cooperative molecular mechanism when designing multi-drug combination therapies.
癌细胞表现出活性氧（ROS）水平升高和氧化还原状态改变。在此，基于这些特性，我们构建了一种来自趋磁细菌AMB-1的多药递送平台AMB@PDAP-Fe（APPF），并实现了磁共振成像（MRI）可视化的肿瘤微环境响应性光热-化学动力学疗法。PDAP-Fe中的铁在肿瘤部位被谷胱甘肽（GSH）还原，并以高活性铁的形式释放，通过芬顿反应催化ROS的产生，从而抑制肿瘤生长。同时，AMB-1细胞中矿化磁小体在近红外区域的显著吸收使其能够有效地将近红外光转化为热能用于光热疗法（PTT），PDAP对此也有贡献。PTT过程中产生的热量加速了铁的释放过程，从而在肿瘤微环境中实现增强的芬顿反应。此外，AMB-1中的磁小体用作MRI造影剂，使治疗过程可视化。这种基于肿瘤微环境响应性趋磁细菌的多药递送平台显示出对抗肿瘤的潜力，并证明了在设计多药联合疗法时理解协同分子机制的实用性和实际意义。
