College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China.
Shanxi Key Laboratory of Earth Surface Processes and Resource Ecology Security in Fenhe River Basin, Taiyuan Normal University, Jinzhong 030619, China.
Genes (Basel). 2024 Aug 23;15(9):1112. doi: 10.3390/genes15091112.
In plants, RopGEF-mediated ROP signaling is pivotal in cellular signaling pathways, including apical growth, pollen germination and perception, intercellular recognition, as well as in responses to biotic and abiotic stresses. In this study, we retrieved a total of 37 RopGEF members from three C Crops, of which 11 are from millet, 11 from sorghum, and 15 from maize. Based on their phylogenetic relationships and structural characteristics, all RopGEF members are classified into four subfamilies. The qRT-PCR technique was utilized to evaluate the expression profiles of 11 across different tissues in foxtail millet. The findings indicated that the majority of the exhibited higher expression levels in leaves as opposed to roots and stems. The levels of expression of genes were examined in response to abiotic stress and plant hormones. , , , and showed significant induction under abiotic stresses such as salt, cold, and heat. On the other hand, , , and were consistently upregulated, while , , , , and were downregulated upon exposure to abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and gibberellic acid (GA) hormones. The alterations in the expression patterns of RopGEF members imply their potential functions in plant growth and development, abiotic stress response, and hormone signal transduction. These discoveries suggest that the genes may function as a potential genetic marker to facilitate future studies in elucidating the functional characteristics of RopGEFs.
在植物中，RopGEF 介导的 ROP 信号在细胞信号通路中至关重要，包括顶端生长、花粉萌发和感知、细胞间识别以及对生物和非生物胁迫的响应。在这项研究中，我们从三种谷子作物中总共检索到 37 个 RopGEF 成员，其中 11 个来自小米，11 个来自高粱，15 个来自玉米。根据它们的系统发育关系和结构特征，所有的 RopGEF 成员分为四个亚家族。qRT-PCR 技术用于评估 11 个在谷子不同组织中的表达谱。结果表明，大多数在叶片中的表达水平高于根和茎。检测了 11 个基因对非生物胁迫和植物激素的响应。结果表明，在盐、冷、热等非生物胁迫下， 、 、 、 显著诱导表达。另一方面， 、 、 和 持续上调，而 、 、 、 和 则在脱落酸（ABA）、乙烯（ET）、水杨酸（SA）和赤霉素（GA）激素处理时下调。RopGEF 成员表达模式的改变表明它们在植物生长发育、非生物胁迫响应和激素信号转导中的潜在功能。这些发现表明， 基因可能作为一个潜在的遗传标记，有助于未来研究阐明 RopGEFs 的功能特征。
