College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China.
Genes (Basel). 2024 Sep 8;15(9):1180. doi: 10.3390/genes15091180.
Beneficial fungi of the genus are among the most widespread biocontrol agents that induce a plant's defense response against pathogens. is one of the main pathogens that can negatively affect production and quality. To investigate the impact of on defense responses to , roots under + (T + F) treatment and (F) treatment were sampled and subjected to transcriptomic analysis. A differential expression analysis revealed that 6361 differentially expressed genes (DEGs) responded to induction. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the 6361 DEGs revealed that the genes significantly clustered into resistance-related pathways, such as the plant-pathogen interaction pathway, phenylpropanoid biosynthesis pathway, flavonoid biosynthesis pathway, isoflavonoid biosynthesis pathway, mitogen-activated protein kinase (MAPK) signaling pathway, and plant hormone signal transduction pathway. Pathway analysis revealed that the , formononetin biosynthesis, biochanin A biosynthesis, and , ROS production, and may be upregulated by and play important roles in disease resistance. Our study further revealed that the HO content was significantly increased by induction. Formononetin and biochanin A had the potential to suppress . Weighted gene coexpression network analysis (WGCNA) revealed one module, including 58 DEGs associated with induction. One core hub gene, , was found to be upregulated by , SA (salicylic acid) and ETH (ethephon). Overall, our data indicate that can induce induced systemic resistance (ISR) and systemic acquired resistance (SAR) in . The results of this study lay a foundation for a further understanding of the molecular mechanism by which induces resistance in .
有益真菌属是最广泛的生物防治剂之一，它能诱导植物对病原体产生防御反应。 是一种主要的病原体，可以对 产生负面影响。为了研究 对 防御反应的影响，在 + （T + F）处理和 （F）处理下取样 根，并进行转录组分析。差异表达分析显示，有 6361 个差异表达基因（DEGs）对 诱导有反应。对这 6361 个 DEGs 的京都基因与基因组百科全书（KEGG）通路富集分析显示，这些基因显著聚类到与抗性相关的通路，如植物-病原体相互作用途径、苯丙烷生物合成途径、类黄酮生物合成途径、异黄酮生物合成途径、丝裂原激活蛋白激酶（MAPK）信号通路和植物激素信号转导途径。通路分析显示， 、芒柄花素生物合成、大豆苷元生物合成、 和 ROS 产生、以及 可能被 诱导而上调，并在抗病性中发挥重要作用。我们的研究进一步表明，HO 含量被 诱导显著增加。芒柄花素和大豆苷元有可能抑制 。加权基因共表达网络分析（WGCNA）显示了一个包含 58 个与 诱导相关的 DEGs 的模块。一个核心枢纽基因 ，被 、SA（水杨酸）和 ETH（乙烯利）诱导而上调。总体而言，我们的数据表明， 可以在 中诱导系统获得性抗性（SAR）和诱导系统抗性（ISR）。这项研究的结果为进一步了解 诱导抗性的分子机制奠定了基础。
