Faculty of Life Science and Technology, Kunming University of Science and Technology, Number 727 Jing Ming South Road, Chenggong District, Kunming, 650500, Yunnan, China.
Yunnan Provincial Key Laboratory of Panax Notoginseng, Kunming, 650500, Yunnan, China.
Genes Genomics. 2019 Dec;41(12):1383-1396. doi: 10.1007/s13258-019-00865-z. Epub 2019 Sep 6.
Panax notoginseng is a famous Chinese herbal medicine, but the root rot disease mainly caused by Fusarium solani severely reduces the yield and quality of its medicinal materials.
The defense priming in P. notoginseng through exogenous application of signaling molecule will supply theoretical support for the exogenous regulation of disease resistance in P. notoginseng.
In this study, the exogenous application of methyl jasmonate (MeJA) increased P. notoginseng's resistance to F. solani. Furthermore, the P. notoginseng transcriptome during F. solani infection was investigated through next-generation sequencing to uncover the resistance mechanism of P. notogingseng induced by MeJA.
The de novo assembly of transcriptome sequences produced 80,551 unigenes, and 36,771 of these unigenes were annotated by at least one database. A differentially expressed gene analysis revealed that a large number of genes related to terpenoid backbone biosynthesis, phenylalanine metabolism, and plant-pathogen interactions were predominantly up-regulated by MeJA. Moreover, jasmonic acid (JA) biosynthesis-related genes and the JA signaling pathway genes, such as linoleate 13S-lipoxygenase, allene oxide cyclase, allene oxide synthase, TIFY, defensin, and pathogenesis-related proteins, showed increased transcriptional levels after inoculation with F. solani. Notably, according to the gene expression analysis, JA and ethylene signaling pathways may act synergistically to positively regulate the defense responses of P. notoginseng to F. solani.
JA signaling appears to play a vital role in P. notoginseng responses to F. solani infection, which will be helpful in improving the disease resistance of P. notoginseng cultivars as well as in developing an environmentally friendly biological control method for root rot disease.
三七是一种著名的中草药，但由尖孢镰刀菌引起的根腐病严重降低了其药材的产量和质量。
通过施加信号分子对三七进行防御激发，为外源调控三七抗病性提供理论支撑。
本研究通过施加茉莉酸甲酯（MeJA）提高了三七对尖孢镰刀菌的抗性。此外，通过下一代测序技术研究了三七在尖孢镰刀菌感染过程中的转录组，以揭示 MeJA 诱导的三七抗性机制。
转录组序列的从头组装产生了 80551 条 unigenes，其中 36771 条 unigenes至少被一个数据库注释。差异表达基因分析表明，大量与萜类骨架生物合成、苯丙氨酸代谢和植物-病原体相互作用相关的基因主要被 MeJA 上调。此外，茉莉酸（JA）生物合成相关基因和 JA 信号通路基因，如亚麻酸 13S-脂氧合酶、丙二烯氧化物环化酶、丙二烯氧化物合酶、TIFY、防御素和病程相关蛋白，在接种尖孢镰刀菌后转录水平升高。值得注意的是，根据基因表达分析，JA 和乙烯信号通路可能协同作用，正向调控三七对尖孢镰刀菌的防御反应。
JA 信号似乎在三七对尖孢镰刀菌感染的反应中起着至关重要的作用，这将有助于提高三七品种的抗病性，并开发一种对根腐病的环保生物防治方法。
