College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China.
Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
Int J Mol Sci. 2024 Sep 20;25(18):10113. doi: 10.3390/ijms251810113.
Perylenequinones (PQs) are important natural compounds that have been extensively utilized in recent years as agents for antimicrobial, anticancer, and antiviral photodynamic therapies. In this study, we investigated the molecular mechanisms regulating PQ biosynthesis by comparing sp. Slf14 with its low PQ titer mutant, Slf14(w). The results indicated that the strain Slf14 exhibited a higher PQ yield, a more vigorous energy metabolism, and a more pronounced oxidation state compared to Slf14(w). Transcriptome analysis consistently revealed that the differences in gene expression between Slf14 and Slf14(w) are primarily associated with genes involved in redox processes and energy metabolism. Additionally, reactive oxygen species (ROS) were shown to play a crucial role in promoting PQ synthesis, as evidenced by the application of ROS-related inhibitors and promoters. Further results demonstrated that mitochondria are significant sources of ROS, which effectively regulate PQ biosynthesis in sp. Slf14. In summary, this research revealed a noteworthy finding: the higher energy metabolism of the strain Slf14 is associated with increased intracellular ROS accumulation, which in turn triggers the activation and expression of gene clusters responsible for PQ synthesis.
邻苯醌（PQs）是一类重要的天然化合物，近年来被广泛应用于抗菌、抗癌和抗病毒光动力治疗。在本研究中，我们通过比较 sp. Slf14 与其低 PQ 产量突变株 Slf14(w)，研究了调控 PQ 生物合成的分子机制。结果表明，与 Slf14(w)相比，菌株 Slf14 表现出更高的 PQ 产量、更旺盛的能量代谢和更明显的氧化状态。转录组分析一致表明，Slf14 和 Slf14(w)之间基因表达的差异主要与涉及氧化还原过程和能量代谢的基因有关。此外，活性氧（ROS）被证明在促进 PQ 合成中起着关键作用，这可以通过 ROS 相关抑制剂和促进剂的应用得到证明。进一步的结果表明，线粒体是 ROS 的重要来源，它有效地调节 sp. Slf14 中的 PQ 生物合成。总之，这项研究揭示了一个值得注意的发现：菌株 Slf14 较高的能量代谢与细胞内 ROS 积累的增加有关，这反过来又触发了负责 PQ 合成的基因簇的激活和表达。
