Bao Zhuanying, Chen Yunni, Zhang Zhibin, Yang Huilin, Yan Riming, Zhu Du
Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, 330022, China.
Key Lab of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.
Appl Microbiol Biotechnol. 2024 May 3;108(1):317. doi: 10.1007/s00253-024-13142-1.
Perylenequinones (PQs) are natural photosensitizing compounds used as photodynamic therapy, and heat stress (HS) is the main limiting factor of mycelial growth and secondary metabolism of fungi. This study aimed to unravel the impact of HS-induced Ca and the calcium signaling pathway on PQ biosynthesis of Shiraia sp. Slf14(w). Meanwhile, the intricate interplay between HS-induced NO and Ca and the calcium signaling pathway was investigated. The outcomes disclosed that Ca and the calcium signaling pathway activated by HS could effectively enhance the production of PQs in Shiraia sp. Slf14(w). Further investigations elucidated the specific mechanism through which NO signaling molecules induced by HS act upon the Ca/CaM (calmodulin) signaling pathway, thus propelling PQ biosynthesis in Shiraia sp. Slf14(w). This was substantiated by decoding the downstream positioning of the CaM/CaN (calcineurin) pathway in relation to NO through comprehensive analyses encompassing transcript levels, enzyme assays, and the introduction of chemical agents. Concurrently, the engagement of Ca and the calcium signaling pathway in heat shock signaling was also evidenced. The implications of our study underscore the pivotal role of HS-induced Ca and the calcium signaling pathway, which not only participate in heat shock signal transduction but also play an instrumental role in promoting PQ biosynthesis. Consequently, our study not only enriches our comprehension of the mechanisms driving HS signaling transduction in fungi but also offers novel insights into the PQ synthesis paradigm within Shiraia sp. Slf14(w). KEY POINTS: • The calcium signaling pathway was proposed to participate in PQ biosynthesis under HS. • HS-induced NO was revealed to act upon the calcium signaling pathway for the first time.
苝醌类化合物（PQs）是用于光动力疗法的天然光敏化合物，热应激（HS）是真菌菌丝体生长和次级代谢的主要限制因素。本研究旨在揭示热应激诱导的钙及钙信号通路对白僵菌Slf14(w)中PQ生物合成的影响。同时，研究了热应激诱导的一氧化氮（NO）与钙及钙信号通路之间复杂的相互作用。结果表明，热应激激活的钙及钙信号通路可有效提高白僵菌Slf14(w)中PQ的产量。进一步研究阐明了热应激诱导的NO信号分子作用于钙/钙调蛋白（CaM）信号通路从而推动白僵菌Slf14(w)中PQ生物合成的具体机制。通过对转录水平、酶活性测定及化学试剂引入等综合分析，解码CaM/钙调神经磷酸酶（CaN）通路相对于NO的下游定位，证实了这一点。同时，也证明了钙及钙信号通路参与热休克信号传导。我们研究的意义强调了热应激诱导的钙及钙信号通路的关键作用，它们不仅参与热休克信号转导，还在促进PQ生物合成中发挥重要作用。因此，我们的研究不仅丰富了我们对真菌中热应激信号转导机制的理解，还为白僵菌Slf14(w)中的PQ合成模式提供了新的见解。要点：• 提出钙信号通路在热应激下参与PQ生物合成。• 首次揭示热应激诱导的NO作用于钙信号通路。
