Xuan Guanhua, Lu Di, Lin Hong, Wang Yinfeng, Wang Jingxue
State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266400, China.
Microorganisms. 2024 Sep 5;12(9):1836. doi: 10.3390/microorganisms12091836.
Several studies have investigated the multifunctional characteristics of outer membrane vesicles (OMVs), but research on their role in mediating phage-bacteria interactions is limited. Employing as a model, we engineered a mutant strain overproducing OMVs for protective experiments against phage infections. The addition of exogenous OMVs proved highly effective in safeguarding the bacterial host against various phages, mitigating predatory threats. Screening for phage-resistant strains and adsorption experiments revealed that inhibiting phage adsorption is a crucial pathway through which OMVs protect against phage predation. Although OMVs conferred tolerance to the phage-sensitive strains (those easily infected by phages), they could not restore the phage-resistant strains (those that effectively resist phage infection) to a sensitive phenotype. This study provides valuable insights for the future development of novel biotechnological approaches aimed at utilizing OMVs to protect fermentative strains and reduce the risk of phage contamination.
多项研究已对外膜囊泡（OMV）的多功能特性进行了调查，但关于其在介导噬菌体与细菌相互作用中作用的研究有限。以[具体内容缺失]为模型，我们构建了一个过量产生OMV的突变菌株，用于针对噬菌体感染的保护性实验。添加外源性OMV被证明在保护细菌宿主免受各种噬菌体侵害、减轻捕食威胁方面非常有效。对噬菌体抗性菌株的筛选和吸附实验表明，抑制噬菌体吸附是OMV抵御噬菌体捕食的关键途径。虽然OMV赋予了噬菌体敏感菌株（那些容易被噬菌体感染的菌株）耐受性，但它们无法使噬菌体抗性菌株（那些有效抵抗噬菌体感染的菌株）恢复到敏感表型。这项研究为未来开发旨在利用OMV保护发酵菌株并降低噬菌体污染风险的新型生物技术方法提供了有价值的见解。
