Koopman B J, Hindriks F R, Lokerse Y G, Wolthers B G, Orverdijk J F
Clin Chem. 1985 Dec;31(12):2030-2.
Colorimetry of iron in serum with Ferrozine (as used in the Technicon SMAC) or with bathophenanthroline (as used in the Du Pont aca) is influenced by EDTA, in contrast to such measurements with atomic absorption spectroscopy. Therefore EDTA contamination should be avoided with these colorimetric methods. If, however, contamination with EDTA is suspected, addition of zinc sulfate to serum or to the SMAC "ascorbic acid reagent" will cancel the influence of EDTA on measurements of iron in the SMAC.
与原子吸收光谱法测定铁不同，使用亚铁嗪（Technicon SMAC所采用）或邻二氮菲（杜邦aca所采用）进行血清中铁的比色测定会受到EDTA的影响。因此，使用这些比色法时应避免EDTA污染。然而，如果怀疑存在EDTA污染，向血清或SMAC“抗坏血酸试剂”中添加硫酸锌将消除EDTA对SMAC中铁测定的影响。
