Ceriotti F, Ceriotti G
Clin Chem. 1980 Feb;26(2):327-31.
Serum iron is released from transferrin and reduced at pH 1.7 by treating serum with a 10 g/L ascorbic acid solution in 0.1 mol/L HCl. When ferrozine is added to this reagent, it forms a complex with iron that is as intensely colored as at higher pH values, and under these conditions no turbidity is produced. The second major interference, that from copper, is eliminated by adding 1 g of thiosemicarbazide per liter, which at a low pH forms a stable, uncolored complex with copper without affecting the reaction of ferrozine with iron.
血清铁从转铁蛋白中释放出来，并在pH 1.7的条件下，通过用0.1 mol/L盐酸中的10 g/L抗坏血酸溶液处理血清来还原。当向该试剂中加入亚铁嗪时，它会与铁形成一种与在较高pH值下颜色一样深的络合物，并且在这些条件下不会产生浑浊。通过每升加入1 g氨基硫脲来消除第二个主要干扰物铜，氨基硫脲在低pH值下与铜形成稳定的无色络合物，而不影响亚铁嗪与铁的反应。
