Keskin Pınar, Kılıç Kanak Eda, Öztürk Yılmaz Suzan
Department of Food Engineering, Sakarya University, Sakarya 54187, Türkiye.
Microorganisms. 2024 Sep 19;12(9):1905. doi: 10.3390/microorganisms12091905.
This research aimed to identify the probiotic features of strains isolated from olive oils in Turkey. The in vitro survival capabilities of strains in gastric and pancreatic solutions were assessed. The hydrophobicity of strains was determined to be between 25.8% and 46.08% for xylene, 22.5% and 45.85% for chloroform, and 14.83% and 37.09% for ethyl acetate. In addition, auto-aggregation values were measured as 11.07-60.35%; 16.28-67.70% and 42.89-85.21% after 2, 4 and 24 h of incubation, respectively. The strains tested in this study demonstrated aggregation ability against the pathogens ATCC 25922, typhimurium ATCC 14028, ATCC 25923 and ATCC 7644. Antibiotic resistance and hemolytic activities were also checked to ensure the safety of the Cholesterol removal by strains ranged from 12.30% to 47.42%, and their free radical scavenging activity varied between 2.85% and 39.10%. Out of 13 samples from 10 different olive oil sources, Y6, Y7, and Y11 exhibited the best strains with probiotic potential properties. This study discovered that with probiotic properties can be isolated in olive oil samples, a finding that has not been previously documented in the literature and may have potential industrial applications.
本研究旨在鉴定从土耳其橄榄油中分离出的菌株的益生菌特性。评估了这些菌株在胃液和胰液中的体外存活能力。测定了这些菌株对二甲苯的疏水性在25.8%至46.08%之间,对氯仿的疏水性在22.5%至45.85%之间,对乙酸乙酯的疏水性在14.83%至37.09%之间。此外,自聚集值在培养2、4和24小时后分别测定为11.07 - 60.35%、16.28 - 67.70%和42.89 - 85.21%。本研究中测试的这些菌株对病原体金黄色葡萄球菌ATCC 25922、鼠伤寒沙门氏菌ATCC 14028、大肠杆菌ATCC 25923和白色念珠菌ATCC 7644表现出聚集能力。还检查了抗生素抗性和溶血活性以确保这些菌株的安全性。这些菌株的胆固醇去除率在12.30%至47.42%之间,其自由基清除活性在2.85%至39.10%之间变化。从10个不同橄榄油来源的13个样品中,Y6、Y7和Y11表现出具有益生菌潜在特性的最佳菌株。本研究发现,具有益生菌特性的菌株可以从橄榄油样品中分离出来,这一发现以前在文献中未曾记载,可能具有潜在的工业应用价值。