Nami Yousef, Vaseghi Bakhshayesh Reza, Mohammadzadeh Jalaly Hossein, Lotfi Hajie, Eslami Solat, Hejazi Mohammad Amin
Department of Food Biotechnology, Branch for Northwest and West Region, Agricultural Biotechnology Research Institute, Education and Extension Organization (AREEO), Tabriz, Iran.
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
Front Microbiol. 2019 Feb 26;10:300. doi: 10.3389/fmicb.2019.00300. eCollection 2019.
The present study focused on probiotic characterization and safety evaluation of isolates from different artisanal dairy products. All the isolates exhibited inhibitory activity against several food spoilage bacteria and food-borne pathogens, including , and . The PCR results indicated the presence of at least one enterocin structural gene in all the tested strains. The isolates were further evaluated regarding their safety properties and functional features. The isolates were susceptible to vancomycin, gentamycin, and chloramphenicol. The results of PCR amplification revealed that all the tested isolates harbored none of the tested virulence genes except (ES9), which showed the presence of gene. The isolates showed cholesterol lowering properties. The selected isolates showed a high tolerance to low pH, and toward bile salts. They also demonstrated hydrophobicity activity, auto-aggregation, and adhesion ability to the human intestinal Caco-2 cell line. These properties may contribute the bacteria colonizing the gut. This study revealed that the isolates, especially ES11, ES20 and ES32, might be excellent candidates for production of functional foods to promote health benefits.
本研究聚焦于来自不同手工乳制品的分离株的益生菌特性及安全性评估。所有分离株均对几种食品腐败菌和食源性病原体表现出抑制活性，包括……。PCR结果表明，所有测试菌株中至少存在一个肠毒素结构基因。对这些分离株的安全性和功能特性进行了进一步评估。这些分离株对万古霉素、庆大霉素和氯霉素敏感。PCR扩增结果显示，除了ES9（其显示存在……基因）外，所有测试分离株均未携带所测试的毒力基因。这些分离株具有降低胆固醇的特性。所选分离株对低pH值和胆盐具有高度耐受性。它们还表现出疏水性、自聚集性以及对人肠道Caco - 2细胞系的黏附能力。这些特性可能有助于细菌在肠道定殖。本研究表明，这些分离株，尤其是ES11、ES20和ES32，可能是生产促进健康功能食品的优良候选菌株。
