Hetzel Carrie A, Appah-Sampong Akua A, Hurst-Manny Austin R, Nibert Max L
Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
Program in Virology, Division of Medical Sciences, Graduate School of Arts and Sciences, Harvard University, Cambridge, MA 02138, USA.
Pathogens. 2024 Aug 29;13(9):733. doi: 10.3390/pathogens13090733.
(Tvag) is a sexually transmitted human pathogen that is commonly infected with strains of one or more of five known species of Trichomonas vaginalis viruses (TVVs), members of genus . TVVs are thought not to have an extracellular phase to their lifecycle and instead to be transmitted vertically from mother to daughter cells. As a result, generation of isogenic virus-positive and virus-negative sets of Tvag clones has been a major barrier to studying interactions between TVVs and their host. Nucleoside analog 2'-C-methylcytidine (2CMC) has been recently reported to clear trichomonads of infections with TVV1, TVV2, and TVV3. We used 2CMC to treat a panel of Tvag isolates that collectively harbor at least one representative strain of each TVV species and thereby provided evidence that infections with TVV4 and TVV5 can also be cleared by 2CMC. Furthermore, our results suggest a newly identified difference in drug susceptibility between TVV species. We took advantage of these susceptibility difference to generate isogenic sets of Tvag clones harboring different combinations of the five TVV species. These results provide both new insight into differences between these species and new avenues for generating tools to study the potential roles of TVVs in Tvag biology.
阴道毛滴虫（Tvag）是一种性传播的人类病原体，通常感染五种已知阴道毛滴虫病毒（TVV）中的一种或多种毒株，TVV属于毛滴虫属。TVV在其生命周期中被认为没有细胞外阶段，而是从母细胞垂直传播到子细胞。因此，产生同基因的病毒阳性和病毒阴性阴道毛滴虫克隆株一直是研究TVV与其宿主之间相互作用的主要障碍。核苷类似物2'-C-甲基胞苷（2CMC）最近被报道可清除感染TVV1、TVV2和TVV3的滴虫。我们使用2CMC处理一组阴道毛滴虫分离株，这些分离株共同包含每个TVV物种的至少一种代表性毒株，从而证明2CMC也可以清除TVV4和TVV5的感染。此外，我们的结果表明TVV物种之间新发现的药物敏感性差异。我们利用这些敏感性差异产生了携带五种TVV物种不同组合的同基因阴道毛滴虫克隆株。这些结果为深入了解这些物种之间的差异以及为研究TVV在阴道毛滴虫生物学中的潜在作用生成工具提供了新途径。
