Park Won-Jong, Bae Gi-Sang, Han Youn-Ho
Department of Oral and Maxillofacial Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
Department of Pharmacology, School of Korean Medicine, Wonkwang University, Iksan 54538, Republic of Korea.
Pathogens. 2024 Sep 4;13(9):761. doi: 10.3390/pathogens13090761.
The influenza A virus poses a serious threat to human health and is an important global public health issue. The drugs currently used for treatment are becoming increasingly ineffective against influenza A viruses and require the development of new antiviral drugs. Angelica tenuissima Nakai (ATN), a traditional herbal medicine belonging to the Umbelliferae family, exhibits a broad range of pharmacological activities, including inflammation, headache, and cold symptoms. In the present study, based on target protein identification, functional enrichment analysis, and gene set comparisons, we first suggested that ATN has potential therapeutic effects against influenza A virus infection. Next, methylthiazol tetrazolium (MTT) and sulforhodamine B colorimetric (SRB) assay results revealed that ATN exhibited low cytotoxicity in Madin-Darby canine kidney (MDCK) cells. The antiviral properties of ATN were observed against H1N1 and H3N2 virus strains. Microscopy confirmed the increased survival rate of the host cells. Further time-of-addition experiments revealed that the addition of ATN before virus adsorption showed similar results to the whole period of treatment. The pre- and co-treated groups showed lower levels of viral RNA (M1 protein). The results of this study suggest that ATN exhibits antiviral properties against the influenza A virus. These therapeutic properties of ATN can serve as a theoretical basis for further research on the applicability of ATN in the development of antiviral agents.
甲型流感病毒对人类健康构成严重威胁，是一个重要的全球公共卫生问题。目前用于治疗的药物对甲型流感病毒的疗效越来越差，因此需要开发新的抗病毒药物。当归（Angelica tenuissima Nakai，ATN）是伞形科的一种传统草药，具有广泛的药理活性，包括抗炎、缓解头痛和感冒症状。在本研究中，基于靶蛋白鉴定、功能富集分析和基因集比较，我们首先提出ATN对甲型流感病毒感染具有潜在的治疗作用。接下来，甲基噻唑四氮唑（MTT）和磺酰罗丹明B比色法（SRB）检测结果显示，ATN在犬肾传代细胞（MDCK）中表现出低细胞毒性。观察到ATN对H1N1和H3N2病毒株具有抗病毒特性。显微镜检查证实宿主细胞的存活率增加。进一步的添加时间实验表明，在病毒吸附前添加ATN与整个治疗期的结果相似。预处理组和共处理组的病毒RNA（M1蛋白）水平较低。本研究结果表明，ATN对甲型流感病毒具有抗病毒特性。ATN的这些治疗特性可为进一步研究ATN在抗病毒药物开发中的适用性提供理论依据。
