Park Yuna, Kim Dayoung, Yang Inho, Choi Bomee, Lee Jin Woo, Namkoong Seung, Koo Hyun Jung, Lee Sung Ryul, Park Myung Rye, Lim Hyosun, Kim Youn Kyu, Nam Sang Jip, Sohn Eun-Hwa
College of Health Science, Kangwon National University, Samcheok 25949, Republic of Korea.
Department of Chemistry and Nano Science, Global Top 5 Program, Ewha Womans University, Seoul 03760, Republic of Korea.
J Microbiol Biotechnol. 2018 Jul 28;28(7):1061-1067. doi: 10.4014/jmb.1812.02044.
The anti-melanogenic effects of the extract of (AT) root and the extract of AT root fermented by (FAT) were investigated. These effects were determined by measuring the inhibitory activity of AT and FAT on melanin production in B16F10 melanocytes and with in vitro tyrosinase activity assays. The AT extract inhibited melanin production at concentrations above 250 μg/ml, and this inhibitory effect was significantly enhanced by the fermentation process with . HPLC analysis resulted in the isolation of two active compounds from both the AT and FAT extracts. Their chemical structures were identified as decursin and Z-ligustilide through comparison with previously reported NMR data. The decursin and Z-ligustilide contents were increased in the FAT extract and could be responsible for its enhanced inhibitory effects on melanin production and tyrosinase activity compared with that of the AT extract.
研究了当归（AT）根提取物和经发酵的当归（FAT）根提取物的抗黑色素生成作用。通过测量AT和FAT对B16F10黑色素细胞中黑色素生成的抑制活性以及进行体外酪氨酸酶活性测定来确定这些作用。当归提取物在浓度高于250μg/ml时抑制黑色素生成，并且通过发酵过程这种抑制作用显著增强。高效液相色谱分析从AT和FAT提取物中分离出两种活性化合物。通过与先前报道的核磁共振数据比较，确定它们的化学结构为紫花前胡素和Z-藁本内酯。与AT提取物相比，FAT提取物中紫花前胡素和Z-藁本内酯的含量增加，这可能是其对黑色素生成和酪氨酸酶活性增强的抑制作用的原因。
