Nguyen Vinh Nghi, Wang San-Lang, Nguyen Thi Huyen, Nguyen Van Bon, Doan Manh Dung, Nguyen Anh Dzung
Ninh Thuan Hospital, Phan Rang-Thap Cham City 59000, Vietnam.
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam.
Polymers (Basel). 2024 Sep 19;16(18):2647. doi: 10.3390/polym16182647.
Chitosan/starch nanocomposites loaded with ampicillin were prepared using the spray-drying method by mixing various ratios of chitosan and starch. The morphology of chitosan/starch nanoparticles was studied using a scanning electron microscope (SEM), and the zeta potential value and size distribution were determined by a Nanoparticle Analyzer. The results show that the chitosan/starch nanocomposites have a spherical shape, smooth surface, and stable structure. Nanoparticle size distribution ranged from 100 to 600 nm, and the average particle size ranged from 300 to 400 nm, depending on the ratio between chitosan and starch. The higher the ratio of starch in the copolymer, the smaller the particle size. Zeta potential values of the nanocomposite were very high, ranging from +54.4 mV to +80.3 mV, and decreased from 63.2 down to +37.3 when loading with ampicillin. The chitosan/starch nanocomposites were also characterized by FT-IR to determine the content of polymers and ampicillin in the nanocomposites. The release kinetics of ampicillin from the nanocomposites were determined in vitro using an HPLC profile for 24 h. The loading efficiency (LE) of ampicillin into chitosan/starch nanoparticles ranged from 75.3 to 77.3%. Ampicillin-loaded chitosan/starch nanocomposites were investigated for their antibacterial activity against antibiotic-resistant in vitro. The results demonstrate that the antibacterial effectiveness of nanochitosan/starch loading with ampicillin against was 95.41%, higher than the 91.40% effectiveness of ampicillin at the same concentration of 5.0 µg/mL after 24 h of treatment. These results suggest that chitosan/starch nanocomposites are potential nanomaterials for antibiotic drug delivery in the pharmaceutical field.
通过混合不同比例的壳聚糖和淀粉，采用喷雾干燥法制备了负载氨苄西林的壳聚糖/淀粉纳米复合材料。使用扫描电子显微镜（SEM）研究了壳聚糖/淀粉纳米颗粒的形态，并通过纳米颗粒分析仪测定了zeta电位值和粒径分布。结果表明，壳聚糖/淀粉纳米复合材料呈球形，表面光滑，结构稳定。纳米颗粒粒径分布在100至600nm之间，平均粒径在300至400nm之间，这取决于壳聚糖和淀粉之间的比例。共聚物中淀粉的比例越高，粒径越小。纳米复合材料的zeta电位值非常高，范围从+54.4mV到+80.3mV，负载氨苄西林后从63.2下降到+37.3。还通过傅里叶变换红外光谱（FT-IR）对壳聚糖/淀粉纳米复合材料进行了表征，以确定纳米复合材料中聚合物和氨苄西林的含量。使用高效液相色谱（HPLC）图谱在体外测定了氨苄西林从纳米复合材料中的释放动力学，持续24小时。氨苄西林负载到壳聚糖/淀粉纳米颗粒中的负载效率（LE）范围为75.3%至77.3%。对负载氨苄西林的壳聚糖/淀粉纳米复合材料进行了体外抗耐药菌抗菌活性研究。结果表明，负载氨苄西林的纳米壳聚糖/淀粉对该菌的抗菌有效性为95.41%，高于相同浓度5.0μg/mL的氨苄西林在处理24小时后的91.40%有效性。这些结果表明，壳聚糖/淀粉纳米复合材料是制药领域抗生素药物递送的潜在纳米材料。
