Nguyen Vinh Nghi, Nguyen Van Bon, Tran Minh Dinh, Doan Manh Dung, Nguyen Dinh Sy, Nguyen Thi Huyen, Doan Chien Thang, Tran Thi Ngoc, Wang San Lang, Nguyen Anh Dzung
Ninh Thuan Hospital, Phan Rang-Thap Cham City, 59000, Viet Nam; Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Viet Nam.
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Viet Nam.
Carbohydr Res. 2024 Nov;545:109274. doi: 10.1016/j.carres.2024.109274. Epub 2024 Sep 13.
Ampicillin (Amp), an antibiotic, is widely used to treat bacterial infections in humans and livestock, but recently the rate of resistance has increased rapidly. The aim of this work was to enhancing the antibacterial effect of this compound against AMR Staphylococcus aureus via loading Amp into chitosan/starch nanocomposites by spray drying technique. The results showed that the different ratio of chitosan gel and starch gel used in preparing the nanocomposites can affect its properties and performance. The size distribution of the nanocomposite particles was ranging from 122.0 to 816.9 nm. The zeta potential values of the nanocomposites range from +29.47 to +93.07 mV, indicating the stability of the particles and their tendency to repel each other. Ampicillin was loaded into the chitosan/starch nanocomposites with encapsulation efficiency of 70.7-77.3 %, then their releasing and antibacterial effect against AMR S. aureus were investigated. The results indicated that antibacterial activity of chitosan/starch nanocomposites loaded ampicillin was much higher than ampicillin alone. Chitosan/starch nanocomposites loaded ampicillin at concentration 5.0 μg/mL inhibited 88.6 % growth of S. aureus to a similar extent as 7.5 μg/mL of ampicillin alone. Additionally, at same 7.5 μg/mL ampicillin concentration, the nanocomposites loaded ampicillin showed a higher inhibitory rate (93.27 %) compared to ampicillin alone (88.96 %) over a 12 h-period. Especially, the antibacterial activity of chitosan/starch nanocomposites loaded ampicillin still maintained their effectiveness over 48 h (95.43 %) while those the ampicillin decreased down to 85.76 %. This research highlights the potential of using the chitosan/starch nanocomposites as nanocarriers for ampicillin to enhance its antibacterial activity against AMR Staphylococcus aureus. This approach could be a promising strategy to combat antimicrobial resistance.
氨苄西林（Amp）是一种抗生素，广泛用于治疗人类和牲畜的细菌感染，但最近耐药率迅速上升。这项工作的目的是通过喷雾干燥技术将氨苄西林负载到壳聚糖/淀粉纳米复合材料中，以增强该化合物对耐甲氧西林金黄色葡萄球菌（AMR S. aureus）的抗菌效果。结果表明，用于制备纳米复合材料的壳聚糖凝胶和淀粉凝胶的不同比例会影响其性能。纳米复合颗粒的尺寸分布在122.0至816.9纳米之间。纳米复合材料的zeta电位值在+29.47至+93.07毫伏之间，表明颗粒的稳定性及其相互排斥的趋势。将氨苄西林负载到壳聚糖/淀粉纳米复合材料中，包封效率为70.7-77.3%，然后研究其对AMR S. aureus的释放和抗菌效果。结果表明，负载氨苄西林的壳聚糖/淀粉纳米复合材料的抗菌活性远高于单独的氨苄西林。浓度为5.0μg/mL的负载氨苄西林的壳聚糖/淀粉纳米复合材料对金黄色葡萄球菌生长的抑制率为88.6%，与单独使用7.5μg/mL氨苄西林的抑制程度相似。此外，在相同的7.5μg/mL氨苄西林浓度下，负载氨苄西林的纳米复合材料在12小时内的抑制率（93.27%）高于单独的氨苄西林（88.96%）。特别是，负载氨苄西林的壳聚糖/淀粉纳米复合材料的抗菌活性在48小时内仍保持有效（95.43%），而单独的氨苄西林则降至85.76%。这项研究突出了使用壳聚糖/淀粉纳米复合材料作为氨苄西林的纳米载体来增强其对AMR金黄色葡萄球菌抗菌活性的潜力。这种方法可能是对抗抗菌药物耐药性的一种有前途的策略。
