NHC Key Laboratory of Tropical Disease Control, Engineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, Hainan Provincial Key Laboratory of Research and Development on Tropical Herbs, School of Tropical Medicine, Hainan Medical University, Haikou 571199, China.
Molecules. 2024 Sep 19;29(18):4446. doi: 10.3390/molecules29184446.
Four new alkaloids, vibripyrrolidine A (), vibripiperazine A (), and vibridiazinane A, B (, ), comprising one pyrrolidine, one piperazine, and two diazinane alkaloids, along with two known analogs (, ), were isolated from the marine bacterium ZXR-93 cultured in ISP2 medium. Their chemical structures were elucidated by analysis of their 1D and 2D NMR, mass spectra, and electronic circular dichroism (ECD) calculations. Compounds and - showed vigorous antibacterial activity against , with MIC values ranging from 0.96 to 7.81 μg/mL. Moreover, compound exhibited robust anti-inflammatory activity in vitro using the LPS-induced RAW264.7 macrophage model. All compounds also showed moderate antineoplastic activity against cervical cancer cells (HeLa) and gastric cancer cells (SGC-7901).
从海洋细菌 ZXR-93 中分离得到的 ISp2 培养基培养物中，分离得到了四个新的生物碱：Vibripyrrolidine A()、Vibripiperazine A()和 Vibridiazinane A、B()，包括一个吡咯烷、一个哌嗪和两个二嗪烷生物碱，以及两个已知的类似物()。通过分析它们的 1D 和 2D NMR、质谱和电子圆二色性（ECD）计算，确定了它们的化学结构。化合物和-对表现出强烈的抗菌活性，对的 MIC 值范围为 0.96 至 7.81 μg/mL。此外，化合物在 LPS 诱导的 RAW264.7 巨噬细胞模型中表现出强大的抗炎活性。所有化合物对宫颈癌细胞（HeLa）和胃癌细胞（SGC-7901）也具有中等的抗肿瘤活性。
