Shad Munsif Ali, Wu Songguo, Rao Muhammad Junaid, Luo Xiaoying, Huang Xiaojin, Wu Yuxin, Zhou Yuhong, Wang Lingqiang, Ma Chongjian, Hu Lihua
Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China.
Plants (Basel). 2024 Sep 13;13(18):2568. doi: 10.3390/plants13182568.
Passion fruit is a valued tropical fruit crop that faces environment-related growth strains. genes are important for both growth modulation and stress prevention in plants. Herein, we systematically analyzed the gene family in passion fruit, recognizing 30 members. Genes exhibiting closer phylogenetic relationships exhibited similar protein and gene structures. Gene members of the family showed developmental-stage- or tissue-specific expression profiles during the passion fruit life cycle. Transcriptome data also demonstrated that many showed induced expression in response to hormonal treatments and cold, heat, and salt stress. Based on transcriptomics data, eight candidate genes were chosen for preferential gene expression confirmation under cold stress conditions. The qRT-PCR assays suggested /// upregulation, while // downregulation after cold stress. Additionally, /// exhibited in silico binding with cold-stress-related miRNA319s. GFP subcellular localization assays exhibited PeTCP19/1 were localized at the nucleus. This study will aid in the establishment of novel germplasm, as well as the further investigation of the roles of and their cold stress resistance characteristics.
西番莲是一种重要的热带水果作物，但面临与环境相关的生长压力。基因对于植物的生长调节和抗逆性都很重要。在此，我们系统地分析了西番莲中的基因家族，识别出30个成员。亲缘关系较近的基因表现出相似的蛋白质和基因结构。该家族的基因成员在西番莲生命周期中呈现出发育阶段或组织特异性的表达模式。转录组数据还表明，许多基因在激素处理以及冷、热和盐胁迫下表现出诱导表达。基于转录组学数据，选择了8个候选基因用于在冷胁迫条件下进行优先基因表达确认。qRT-PCR分析表明///在冷胁迫后上调，而//下调。此外，///在计算机模拟中显示与冷胁迫相关的miRNA319s结合。GFP亚细胞定位分析表明PeTCP19/1定位于细胞核。本研究将有助于建立新的种质资源，并进一步研究基因及其抗寒特性的作用。
