He Bei, Zhou Yuan, Peng Yu, Xu Dongyun, Tong Jun, Dong Yanfang, Fang Linchuan, Mao Jing
Institute of Forestry and Fruit Tree, Wuhan Academy of Agricultural Sciences, Wuhan 430070, China.
Horticulture and Forestry College, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2024 Sep 13;13(18):2569. doi: 10.3390/plants13182569.
Rhododendron, with its high ornamental value and ecological benefits, is severely impacted by the azalea lace bug (), one of its primary pests. This study utilized three Rhododendron cultivars, 'Zihe', 'Yanzhimi', and 'Taile', to conduct a non-targeted metabolomic analysis of leaf samples before and after azalea lace bug stress using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME/GCMS) and liquid chromatography-mass spectrometry (LCMS). A total of 81 volatile metabolites across 11 categories and 448 nonvolatile metabolites across 55 categories were detected. Significant differences in metabolic profiles were observed among the different cultivars after pest stress. A total of 47 volatile compounds and 49 nonvolatile metabolites were upregulated in the most susceptible cultivar 'Zihe', including terpenes, alcohols, nucleotides, amino acids, and carbohydrates, which are involved in energy production and secondary metabolism. Conversely, 'Yanzhimi' showed a downtrend in both the differential volatiles and metabolites related to purine metabolism and zeatin biosynthesis under pest stress. The resistant cultivar 'Taile' exhibited moderate changes, with 17 volatile compounds and 17 nonvolatile compounds being upregulated and enriched in the biosynthesis of amino acids, pentose, glucuronate interconversions, carbon metabolism, etc. The phenylalanine metabolic pathway played an important role in the pest resistance of different susceptible cultivars, and relevant metabolites such as phenylethyl alcohol, methyl salicylate, and apigenin may be involved in the plant's resistance response. The results of this study provide a new perspective on the metabolomics of Rhododendron-insect interactions and offer references for the development of pest control strategies.
杜鹃花具有较高的观赏价值和生态效益，但受到其主要害虫之一杜鹃网蝽的严重影响。本研究利用‘紫荷’‘胭脂蜜’和‘泰乐’三个杜鹃品种，采用顶空固相微萃取结合气相色谱 - 质谱联用仪（HS - SPME/GCMS）和液相色谱 - 质谱联用仪（LCMS），对杜鹃网蝽胁迫前后的叶片样本进行非靶向代谢组学分析。共检测到11类81种挥发性代谢物和55类448种非挥发性代谢物。害虫胁迫后，不同品种间代谢谱存在显著差异。在最易感品种‘紫荷’中，共有47种挥发性化合物和49种非挥发性代谢物上调，包括萜类、醇类、核苷酸、氨基酸和碳水化合物，它们参与能量产生和次生代谢。相反，‘胭脂蜜’在害虫胁迫下，与嘌呤代谢和玉米素生物合成相关的差异挥发性化合物和代谢物均呈下降趋势。抗性品种‘泰乐’表现出适度变化，有17种挥发性化合物和17种非挥发性化合物上调，并在氨基酸生物合成、戊糖、葡萄糖醛酸互变、碳代谢等过程中富集。苯丙氨酸代谢途径在不同易感品种的抗虫性中起重要作用，苯乙醇、水杨酸甲酯和芹菜素等相关代谢物可能参与植物的抗性反应。本研究结果为杜鹃花 - 昆虫相互作用的代谢组学提供了新视角，为害虫防治策略的制定提供了参考。
