Key Laboratory of Plant Physiology and Development Regulation, Guizhou Normal University, Guiyang 550025, China.
Key Laboratory of Environment Friendly Management on Alpine Rhododendron Diseases and Pests of Institutions of Higher Learning in Guizhou Province, Guizhou Normal University, Guiyang 550025, China.
Int J Mol Sci. 2023 Jun 22;24(13):10509. doi: 10.3390/ijms241310509.
Climate change has resulted in frequent heavy and prolonged rainfall events that exacerbate waterlogging stress, leading to the death of certain alpine trees. To shed light on the physiological and molecular mechanisms behind waterlogging stress in woody trees, we conducted a study of , a well-known alpine flower species. Specifically, we investigated the physiological and molecular changes that occurred in leaves of subjected to 30 days of waterlogging stress (WS30d), as well as subsequent post-waterlogging recovery period of 10 days (WS30d-R10d). Our findings reveal that waterlogging stress causes a significant reduction in CO assimilation rate, stomatal conductance, transpiration rate, and maximum photochemical efficiency of PSII (Fv/Fm) in the WS30d leaves, by 91.2%, 95.3%, 93.3%, and 8.4%, respectively, when compared to the control leaves. Furthermore, the chlorophyll a and total chlorophyll content in the WS30d leaves decreased by 13.5% and 16.6%, respectively. Both WS30d and WS30d-R10d leaves exhibited excessive HO accumulation, with a corresponding decrease in lignin content in the WS30d-R10d leaves. At the molecular level, purine metabolism, glutathione metabolism, photosynthesis, and photosynthesis-antenna protein pathways were found to be primarily involved in WS30d leaves, whereas phenylpropanoid biosynthesis, fatty acid metabolism, fatty acid biosynthesis, fatty acid elongation, and cutin, suberin, and wax biosynthesis pathways were significantly enriched in WS30d-R10d leaves. Additionally, both WS30d and WS30d-R10d leaves displayed a build-up of sugars. Overall, our integrated transcriptomic, physiological, and metabolomic analysis demonstrated that is susceptible to waterlogging stress, which causes irreversible detrimental effects on both its physiological and molecular aspects, hence compromising the tree's ability to fully recover, even under normal growth conditions.
气候变化导致频繁的强降雨和长时间降雨事件加剧了水涝胁迫，导致某些高山树木死亡。为了揭示木本植物水涝胁迫的生理和分子机制，我们对一种著名的高山花卉 进行了研究。具体来说，我们研究了在水涝胁迫 30 天（WS30d）以及随后的 10 天水涝后恢复期间（WS30d-R10d）叶片中发生的生理和分子变化。我们的研究结果表明，与对照叶片相比，水涝胁迫导致 WS30d 叶片的 CO 同化率、气孔导度、蒸腾速率和 PSII 最大光化学效率（Fv/Fm）分别显著降低了 91.2%、95.3%、93.3%和 8.4%。此外，WS30d 叶片中的叶绿素 a 和总叶绿素含量分别下降了 13.5%和 16.6%。WS30d 和 WS30d-R10d 叶片均表现出过量的 HO 积累，相应地，WS30d-R10d 叶片中的木质素含量下降。在分子水平上，嘌呤代谢、谷胱甘肽代谢、光合作用和光合作用天线蛋白途径被发现主要参与 WS30d 叶片，而苯丙烷生物合成、脂肪酸代谢、脂肪酸生物合成、脂肪酸延长和角质、栓质和蜡生物合成途径在 WS30d-R10d 叶片中显著富集。此外，WS30d 和 WS30d-R10d 叶片均显示出糖的积累。总的来说，我们的综合转录组学、生理学和代谢组学分析表明，对水涝胁迫敏感，这对其生理和分子方面都造成了不可逆转的有害影响，从而削弱了树木完全恢复的能力，即使在正常生长条件下也是如此。
