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转录组学和代谢组学数据揭示了参与苯丙烷代谢途径并调节映山红花香的关键基因。

Transcriptomic and metabolomic data reveal key genes that are involved in the phenylpropanoid pathway and regulate the floral fragrance of Rhododendron fortunei.

机构信息

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, Zhejiang, China.

Ningbo Forest Farm, Ningbo, 315100, Zhejiang, China.

出版信息

BMC Plant Biol. 2023 Jan 5;23(1):8. doi: 10.1186/s12870-022-04016-7.

DOI:10.1186/s12870-022-04016-7
PMID:36600207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9814181/
Abstract

BACKGROUND

To reveal the key genes involved in the phenylpropanoid pathway, which ultimately governs the fragrance of Rhododendron fortunei, we performed a comprehensive transcriptome and metabolomic analysis of the petals of two different varieties of two alpine rhododendrons: the scented R. fortunei and the unscented Rhododendron 'Nova Zembla'.

RESULTS

Our transcriptomic and qRT-PCR data showed that nine candidate genes were highly expressed in R. fortunei but were downregulated in Rhododendron 'Nova Zembla'. Among these genes, EGS expression was significantly positively correlated with various volatile benzene/phenylpropanoid compounds and significantly negatively correlated with the contents of various nonvolatile compounds, whereas CCoAOMT, PAL, C4H, and BALDH expression was significantly negatively correlated with the contents of various volatile benzene/phenylpropanoid compounds and significantly positively correlated with the contents of various nonvolatile compounds. CCR, CAD, 4CL, and SAMT expression was significantly negatively correlated with the contents of various benzene/phenylpropanoid compounds. The validation of RfSAMT showed that the RfSAMT gene regulates the synthesis of aromatic metabolites in R. fortunei.

CONCLUSION

The findings of this study indicated that key candidate genes and metabolites involved in the phenylpropanoid biosynthesis pathway may govern the fragrance of R. fortunei. This lays a foundation for further research on the molecular mechanism underlying fragrance in the genus Rhododendron.

摘要

背景

为了揭示参与苯丙烷途径的关键基因，该途径最终控制了映山红的香气，我们对两种高山映山红（有香味的映山红和无味的 Rhododendron 'Nova Zembla'）的花瓣进行了全面的转录组和代谢组分析。

结果

我们的转录组和 qRT-PCR 数据显示，在有香味的映山红中，有 9 个候选基因表达水平较高，但在 Rhododendron 'Nova Zembla'中则下调。在这些基因中，EGS 的表达与各种挥发性苯/苯丙烷化合物显著正相关，与各种非挥发性化合物的含量显著负相关，而 CCoAOMT、PAL、C4H 和 BALDH 的表达与各种挥发性苯/苯丙烷化合物的含量显著负相关，与各种非挥发性化合物的含量显著正相关。CCR、CAD、4CL 和 SAMT 的表达与各种苯/苯丙烷化合物的含量显著负相关。RfSAMT 的验证表明，RfSAMT 基因调节了有香味的映山红中芳香代谢物的合成。

结论

本研究的结果表明，参与苯丙烷生物合成途径的关键候选基因和代谢物可能控制了映山红的香气。这为进一步研究映山红属香气的分子机制奠定了基础。

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