Fan Li, Zhang Chen, Li Jiafeng, Zhao Zhongtao, Liu Yan
Horticultural and Crop Protection College, Inner Mongolia Agricultural University, Hohhot 010018, China.
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510520, China.
Plants (Basel). 2024 Sep 22;13(18):2652. doi: 10.3390/plants13182652.
Several studies have been devoted to seeking some beneficial plant-related microorganisms for a long time, and on this basis, it has been found that arbuscular mycorrhizal fungi (AMF) have a considerable positive impact on plant health as a biological fungal agent. In this study, we focused on the effects of different AMF on the growth dynamics and root configuration of licorice under saline and alkali conditions. The metabolites of licorice under different AMF were assessed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). (Fm) and (Ri) were added as different AMF treatments, while the sterilized saline-alkali soil was treated as a control. Samples were taken in the R1 period (15 d after AMF treatment) and the R2 period (45 d after AMF treatment). The results showed that the application of AMF significantly increased the root growth of licorice and significantly increased the biomass of both shoot and root. A total of 978 metabolites were detected and divided into 12 groups including lipids, which accounted for 15.44%; organic acids and their derivatives, at 5.83%; benzene compounds and organic heterocyclic compounds, at 5.42%; organic oxides, at 3.78%; and ketones, accounting for 3.17%. Compared with the control, there were significant changes in the differential metabolites with treatment inoculated with AMF; the metabolic pathways and biosynthesis of secondary metabolites were the main differential metabolite enrichment pathways in the R1 period, and those in the R2 period were microbial metabolism in diverse environments and the degradation of aromatic compounds. In conclusion, the use of AMF as biofertilizer can effectively improve the growth of licorice, especially in terms of the root development and metabolites, in saline-alkali soil conditions.
长期以来，已有多项研究致力于寻找一些有益的植物相关微生物，在此基础上，人们发现丛枝菌根真菌（AMF）作为一种生物真菌剂，对植物健康具有相当大的积极影响。在本研究中，我们重点关注了不同AMF对盐碱条件下甘草生长动态和根系构型的影响。使用液相色谱-串联质谱（LC-MS/MS）对不同AMF处理下甘草的代谢产物进行了评估。添加了（Fm）和（Ri）作为不同的AMF处理，同时将灭菌后的盐碱土作为对照。在R1期（AMF处理后15天）和R2期（AMF处理后45天）采集样本。结果表明，AMF的施用显著促进了甘草根系生长，显著增加了地上部和根部的生物量。共检测到978种代谢产物，分为12组，包括脂质，占15.44%；有机酸及其衍生物，占5.83%；苯化合物和有机杂环化合物，占5.42%；有机氧化物，占3.78%；以及酮类，占3.17%。与对照相比，接种AMF处理后的差异代谢产物有显著变化；R1期差异代谢产物的主要富集途径是次生代谢产物的代谢途径和生物合成，R2期则是不同环境中的微生物代谢和芳香化合物的降解。总之，在盐碱土条件下，使用AMF作为生物肥料可以有效促进甘草生长，特别是在根系发育和代谢产物方面。
