College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Xinjiang Production & Construction Corps Key Laboratory of Animal Biomedicine, Tumushuke 843900, China.
Viruses. 2024 Aug 23;16(9):1350. doi: 10.3390/v16091350.
The highly abundant and stable antiviral small RNA derived from honeysuckle, known as miR2911, has been shown to play a key role in inhibiting influenza virus infection and SARS-CoV-2 infection. However, whether miR2911 inhibits the replication of porcine reproductive and respiratory syndrome virus (PRRSV) remains unknown. Hence, this study investigated the mechanisms underlying the action of miR2911 during PRRSV infection. Six targets of miR2911 within the PRRSV orf1 (Nsp2: 2459 to 2477, 1871 to 1892, 954 to 977, and 1271 to 1292; Nsp1: 274 to 296 and 822 to 841) were successfully identified by using the miRanda v1.0b software. The miR2911 target sequence was analyzed by target sequence comparison, and only individual base mutations existed in different prevalent strains, and the miR2911 target region was highly conserved among different strains. Subsequently, through the dual luciferase reporter gene assay and miR2911 overexpression assay, it was demonstrated that miR2911 significantly inhibits the replication of PRRSV by targeting regions of PRRSV Nsp1 and Nsp2. These findings offer new insights for the development of novel anti-PRRSV drugs.
从金银花中提取的高度丰富且稳定的抗病毒小 RNA，即 miR2911，已被证明在抑制流感病毒感染和 SARS-CoV-2 感染方面发挥着关键作用。然而，miR2911 是否抑制猪繁殖与呼吸综合征病毒（PRRSV）的复制尚不清楚。因此，本研究探讨了 miR2911 在 PRRSV 感染过程中的作用机制。使用 miRanda v1.0b 软件成功鉴定了 PRRSV orf1 中 miR2911 的六个靶标（Nsp2：2459 到 2477、1871 到 1892、954 到 977 和 1271 到 1292；Nsp1：274 到 296 和 822 到 841）。通过靶序列比较分析了 miR2911 的靶序列，仅在不同流行株中存在个别碱基突变，并且不同株之间的 miR2911 靶区高度保守。随后，通过双荧光素酶报告基因检测和 miR2911 过表达检测，证明 miR2911 通过靶向 PRRSV Nsp1 和 Nsp2 的区域显著抑制 PRRSV 的复制。这些发现为开发新型抗 PRRSV 药物提供了新的思路。
