Lampe M F, Bott K F
Gene. 1985;38(1-3):139-44. doi: 10.1016/0378-1119(85)90212-4.
A cloned DNA fragment from the ori region of the Bacillus subtilis chromosome permits three separate recF mutants to grow in the presence of mitomycin C (MC) and survive after ultraviolet (UV) exposure. The recF gene has been localized to a 2.3-kb EcoRI-SalI restriction fragment on the cloned sequences. This fragment directs expression of a 34.7-kDal protein in Escherichia coli maxicells. Cloned DNA containing the recF gene does not complement or rescue recL, recM or spoOJ B. subtilis mutants. The B. subtilis recF gene also does not complement any of several of the E. coli recombination-deficient (Rec-) mutants tested.
来自枯草芽孢杆菌染色体ori区域的一个克隆DNA片段，能使三个不同的recF突变体在丝裂霉素C（MC）存在的情况下生长，并在紫外线（UV）照射后存活。recF基因已定位到克隆序列上一个2.3kb的EcoRI-SalI限制片段。该片段在大肠杆菌大细胞中指导一种34.7kDal蛋白质的表达。含有recF基因的克隆DNA不能互补或拯救枯草芽孢杆菌recL、recM或spoOJ突变体。枯草芽孢杆菌recF基因也不能互补所测试的几种大肠杆菌重组缺陷（Rec-）突变体中的任何一种。
