Gavrilova E V, Mekhedov S L, Prozorov A A, Khasanov F K
Genetika. 1992 May;28(5):29-39.
Chromosomal DNA fragment which complemented rec223 mutation of Bacillus subtilis was cloned. Introduction of one copy of the cloned gene into the cells of the rec mutant restored both normal activity for DNA damages repair after mitomycin C action and recombination proficiency. Using multicopy vector led to no formation of recombinants, which was probably connected with overproduction of rec223 gene protein product in Bacillus subtilis cells.
克隆了能互补枯草芽孢杆菌rec223突变的染色体DNA片段。将一份克隆基因导入rec突变体细胞后，恢复了丝裂霉素C作用后DNA损伤修复的正常活性以及重组能力。使用多拷贝载体未导致重组体形成，这可能与枯草芽孢杆菌细胞中rec223基因蛋白产物的过量产生有关。
