Bolat İsmail, Bolat Merve, Kiliçlioğlu Metin, Okur Sıtkıcan, Gölgeli Ayşe, Gözegir Berrah, Çomakli Selim, Yildirim Serkan, Sağlam Yavuz Selim, Warda Mohamad
Department of Pathology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.
Department of Physiology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.
Biol Trace Elem Res. 2025 Jun;203(6):3181-3194. doi: 10.1007/s12011-024-04400-4. Epub 2024 Sep 28.
Post-operative intra-abdominal adhesions, significantly affecting testicular tissue, are a prevalent and serious complication following laparoscopic surgery. This study investigated the efficacy of boric acid, known for its antioxidant, anti-inflammatory, and anti-apoptotic properties, in preventing post-operative testicular adhesions. Forty rats, were divided into four groups: control, laparoscopy (LA), boric acid (BA), and LA + BA. Following laparoscopic surgery, BA treatment was administered for seven days. While the adhesion score was around 3 in the LA group, it was 1 or below in the LA + BA group. Testicular tissues were examined by histopathological and biochemical methods. In testis tissues, in the LA group, malondialdehyde (MDA) levels increased while superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels decreased; these parameters normalized with BA treatment. Additionally, the LA group exhibited reduced levels of IL-10, Bcl-2, Kisspeptin-1, and GnRH, alongside elevated levels of inflammatory markers IL-1β, IL-6, TNF-α, JNK, BAX, and Caspase 3. BA treatment significantly restored these levels to normal. In conclusion, oxidative stress, inflammation, and apoptosis in testicular tissues were associated with post-operative testicular adhesions. BA demonstrated potential as an anti-adhesive agent, reducing testicular adhesions and normalizing biochemical and histological parameters following laparoscopic surgery.
术后腹腔粘连是腹腔镜手术后常见且严重的并发症，对睾丸组织有显著影响。本研究调查了以其抗氧化、抗炎和抗凋亡特性而闻名的硼酸在预防术后睾丸粘连方面的疗效。40只大鼠被分为四组：对照组、腹腔镜手术组（LA）、硼酸组（BA）和LA + BA组。腹腔镜手术后，给予BA治疗7天。LA组的粘连评分约为3，而LA + BA组的粘连评分在1或以下。通过组织病理学和生化方法检查睾丸组织。在睾丸组织中，LA组丙二醛（MDA）水平升高，而超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）水平降低；经BA治疗后这些参数恢复正常。此外，LA组白细胞介素-10（IL-10）、B细胞淋巴瘤-2（Bcl-2）、亲吻素-1（Kisspeptin-1）和促性腺激素释放激素（GnRH）水平降低，同时炎症标志物白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、c-Jun氨基末端激酶（JNK）、Bax蛋白和半胱天冬酶-3（Caspase 3）水平升高。BA治疗显著使这些水平恢复正常。总之，睾丸组织中的氧化应激、炎症和凋亡与术后睾丸粘连有关。BA显示出作为抗粘连剂的潜力，可减少腹腔镜手术后的睾丸粘连，并使生化和组织学参数恢复正常。
