Sulukan Ekrem, Baran Alper, Şenol Onur, Kankaynar Meryem, Yıldırım Serkan, Bolat İsmail, Ceyhun Hacer Akgül, Toraman Emine, Ceyhun Saltuk Buğrahan
Aquatic Biotechnology Laboratory, Fisheries Faculty, Atatürk University, Erzurum, Turkey; Aquaculture Department, Fisheries Faculty, Atatürk University, Erzurum, Turkey; Department of Aquaculture Engineering, Graduate School of Natural and Applied Science, Atatürk University, Erzurum, Turkey.
Aquatic Biotechnology Laboratory, Fisheries Faculty, Atatürk University, Erzurum, Turkey; Department of Food Quality Control and Analysis, Technical Vocational School, Atatürk University, Erzurum, Turkey.
Sci Total Environ. 2023 Feb 1;858(Pt 3):160086. doi: 10.1016/j.scitotenv.2022.160086. Epub 2022 Nov 8.
Global warming further increases the toxic threat of environmental pollutants on organisms. In order to reveal the dimensions of this threat more clearly, it is of great importance that the studies be carried out with temperature differences as close as possible to the temperature values that will represent the global climate projection. In our study, how the toxicity of glyphosate, which is widely used around the world, on zebrafish changes with temperature increases of 0.5° was investigated on behavioral and molecular basis. For this purpose, adult zebrafish were exposed to glyphosate at concentrations of 1 ppm and 5 ppm for 96 h in four environments with a temperature difference of 0.5° (28.5; 29.0; 29.5; 30.0 °C). At the end of the exposure, half of the zebrafish were sampled and remaining half were left for a 10-day recovery process. At the end of the trials, zebrafish were subjected to circadian rhythm and anxiety tests. In addition, histopathological, immunohistochemical and metabolome analyses were performed on brain tissues. As a result, it has been detected that anxiety and circadian rhythm were disrupted in parallel with the increased temperature and glyphosate concentration, and increased histopathological findings and 5-HT4R and GNAT2 immunopositivity in the brain. As a result of metabolome analysis, more than thirty annotated metabolites have been determined due to the synergistic effect of temperature increase and glyphosate exposure. As a conclusion, it was concluded that even a temperature increase of 0.5° caused an increasing effect of glyphosate toxicity in the zebrafish model.
全球变暖进一步加剧了环境污染物对生物的毒性威胁。为了更清楚地揭示这种威胁的程度，以尽可能接近代表全球气候预测的温度值的温差进行研究非常重要。在我们的研究中，从行为和分子层面研究了全球广泛使用的草甘膦对斑马鱼的毒性如何随温度升高0.5°而变化。为此，将成年斑马鱼在温差为0.5°（28.5；29.0；29.5；30.0°C）的四种环境中，分别暴露于浓度为1 ppm和5 ppm的草甘膦中96小时。暴露结束时，对一半的斑马鱼进行采样，另一半则留作10天的恢复过程。试验结束时，对斑马鱼进行昼夜节律和焦虑测试。此外，对脑组织进行了组织病理学、免疫组织化学和代谢组分析。结果发现，焦虑和昼夜节律随着温度和草甘膦浓度的增加而受到干扰，脑组织中的组织病理学结果以及5-HT4R和GNAT2免疫阳性增加。代谢组分析结果显示，由于温度升高和草甘膦暴露的协同作用，已确定了三十多种注释代谢物。总之，得出的结论是，即使温度仅升高0.5°，也会导致斑马鱼模型中草甘膦毒性的增强效应。
