Lenoir G M, Vuillaume M, Bonnardel C
IARC Sci Publ. 1985(60):309-18.
To better characterize the virological and cytogenetic features of Burkitt's lymphoma (BL) occurring in so-called low-incidence areas, biopsy specimens were collected from BL patients diagnosed and treated in France, and attempts were made to cultivate malignant cells in vitro in order to provide large amounts of tumour material for further laboratory investigations. Sixty new BL-derived lymphomatous cell lines have been established from 43 individuals: 24 Caucasians, 14 North Africans and five Africans. Of these, 27 lines (from 18 individuals) were established from non-Epstein-Barr virus (EBV)-associated BL tumours, indicating that the relative ease in cultivating BL malignant cells in vitro is not limited to EBV genome-containing BL tumours. Cytogenetic investigations showed that all the BL cell lines had one of the three 'BL translocations' - t(8;14), t(8;22) or t(2;8). An estimate of the frequency of each translocation, based on the analysis of 51 independent IARC BL cell lines gave the following results: t(8;14), 76%; t(8;22), 16%; t(2;8), 8%. This large panel of cell lines is now a valuable tool for analysing the various phenotypic characteristics of BL cells. Comparisons can be made between multiple lines of BL from high-, intermediate- and low-incidence areas; significant numbers of EBV genome-negative and -positive lines and of tumour cells taken at diagnosis and at various stages of the disease can also be compared. For molecular and immunological investigations, EBV-immortalized lymphoblastoid cell lines (LCL) were also established from non-malignant lymphocytes of BL patients. The 24 paired BL and LCL cell lines obtained so far will allow precise, controlled studies of the molecular consequences of chromosomal translocations and of the comparative susceptibility of BL and LCL to immune effectors.
为了更好地描述在所谓低发病率地区发生的伯基特淋巴瘤（BL）的病毒学和细胞遗传学特征，我们从在法国诊断和治疗的BL患者身上采集了活检标本，并尝试在体外培养恶性细胞，以便为进一步的实验室研究提供大量肿瘤材料。已从43名个体中建立了60个新的源自BL的淋巴瘤细胞系：24名高加索人、14名北非人以及5名非洲人。其中，27个细胞系（来自18名个体）是从非爱泼斯坦-巴尔病毒（EBV）相关的BL肿瘤中建立的，这表明在体外培养BL恶性细胞相对容易的情况并不局限于含有EBV基因组的BL肿瘤。细胞遗传学研究表明，所有BL细胞系都具有三种“BL易位”之一——t(8;14)、t(8;22)或t(2;8)。基于对51个独立的国际癌症研究机构（IARC）BL细胞系的分析，对每种易位频率的估计得出以下结果：t(8;14)，76%；t(8;22)，16%；t(2;8)，8%。这一大组细胞系现在是分析BL细胞各种表型特征的宝贵工具。可以在高、中、低发病率地区的多个BL细胞系之间进行比较；也可以比较大量EBV基因组阴性和阳性细胞系以及在疾病诊断时和不同阶段获取的肿瘤细胞。为了进行分子和免疫学研究，还从BL患者的非恶性淋巴细胞中建立了EBV永生化淋巴母细胞系（LCL）。目前获得的24对BL和LCL细胞系将允许对染色体易位的分子后果以及BL和LCL对免疫效应器的相对敏感性进行精确、可控的研究。
