Sakthivel Shruthi, Thangavel Ponrasu, Saravanakumar Iniyan, Muthuvijayan Vignesh
Tissue Engineering and Biomaterials Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600036, India.
Chem Asian J. 2024 Dec 16;19(24):e202400839. doi: 10.1002/asia.202400839. Epub 2024 Nov 8.
An antioxidant, antibacterial, and biocompatible biomaterial is essential to repair skin wounds effectively. Here, we have employed two natural biopolymers, isabgol (ISAB) and konjac glucomannan (KGM), to prepare microporous scaffolds by freezing and lyophilization. The scaffolds are loaded with thymol (THY) to impart potent antioxidant and antibacterial activities. The physicochemical properties of the ISAB+KGM+THY scaffold, like porosity (41.8±2.4 %), swelling, and biodegradation, were optimal for tissue regeneration application. Compared to the control, ISAB+KGM+THY scaffolds promote attachment, migration, and proliferation of L929 fibroblasts. The antioxidant activity of the ISAB+KGM+THY scaffold was significantly improved after loading THY. This would protect the tissues from oxidative damage. The antibacterial activity of the ISAB+KGM+THY scaffold was significantly higher than that of the control, which would help prevent bacterial infection. The vascularization ability of the ISAB+KGM scaffold was not altered by incorporating THY in the ISAB+KGM scaffold. Therefore, a strong antioxidant, antibacterial, and biocompatible nature of the ISAB+KGM+THY scaffold could be useful for various biomedical applications.
一种具有抗氧化、抗菌和生物相容性的生物材料对于有效修复皮肤伤口至关重要。在此，我们采用了两种天然生物聚合物，即胡芦巴（ISAB）和魔芋葡甘露聚糖（KGM），通过冷冻和冻干制备微孔支架。这些支架负载百里香酚（THY）以赋予强大的抗氧化和抗菌活性。ISAB+KGM+THY支架的物理化学性质，如孔隙率（41.8±2.4 %）、膨胀和生物降解，对于组织再生应用而言是最佳的。与对照组相比，ISAB+KGM+THY支架促进L929成纤维细胞的附着、迁移和增殖。负载THY后，ISAB+KGM+THY支架的抗氧化活性显著提高。这将保护组织免受氧化损伤。ISAB+KGM+THY支架的抗菌活性显著高于对照组，这将有助于预防细菌感染。在ISAB+KGM支架中加入THY不会改变其血管生成能力。因此，ISAB+KGM+THY支架强大的抗氧化、抗菌和生物相容性特性可用于各种生物医学应用。
