Xu Xiaowei, Yong Jie, Wang Lin, Chen Su, Pang Jie
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
State Key Laboratory of Materials-Oriented Chemical Engineering, Jiangsu Key Laboratory of Fine Chemicals and Functional Polymer Materials, Nanjing Tech University, Nanjing 210009, PR China; College of Chemical Engineering, Jiangsu Key Laboratory of Fine Chemicals and Functional Polymer Materials, Nanjing Tech University, Nanjing 210009, PR China.
Int J Biol Macromol. 2025 Apr;304(Pt 1):140198. doi: 10.1016/j.ijbiomac.2025.140198. Epub 2025 Jan 31.
The aim of this study was to utilize pH-responsive hydrogel skin scaffolds to achieve the sustained release of bioactive compounds (BCs) to promote wound healing. ZIF-8@flavanone nanoparticles were successfully synthesized through microfluidic channels and integrated into a hydrogel scaffold consisting of carrageenan (KC) and konjac glucomannan (KGM) to create a pH-responsive composite hydrogel scaffold. The unique pore structure of ZIF-8 efficiently loaded the active material, and the coordination bonds were disrupted in a slightly acidic environment, allowing the release of flavonoids. The natural macromolecular polysaccharides KC and KGM have good biocompatibility, and as a moist but not wet hydrogel scaffold substrate, they more closely resemble the growth environment of cells and are more favorable for wound healing. The hydrogel scaffolds developed in this study had a tensile strength of 1.15 MPa, an elongation at break of 320 %, good biocompatibility, a relative cell viability of 99.6 %, relative antimicrobial rate of 96.3 % (E. coli) and 95.8 % (S. aureus), respectively, and a release rate of 43.3 % at a pH of 5.0, and a wound closure rate at 15 d of 90.5 %, contributing to the improvement of wound healing performance and providing new possibilities for customized antimicrobial wound dressings.
本研究的目的是利用pH响应性水凝胶皮肤支架实现生物活性化合物（BCs）的持续释放，以促进伤口愈合。通过微流控通道成功合成了ZIF-8@黄酮纳米颗粒，并将其整合到由角叉菜胶（KC）和魔芋葡甘露聚糖（KGM）组成的水凝胶支架中，以创建一种pH响应性复合水凝胶支架。ZIF-8独特的孔结构有效地负载了活性物质，并且配位键在微酸性环境中被破坏，从而使黄酮类化合物得以释放。天然高分子多糖KC和KGM具有良好的生物相容性，作为一种湿润但不潮湿的水凝胶支架基质，它们更类似于细胞的生长环境，更有利于伤口愈合。本研究开发的水凝胶支架的拉伸强度为1.15 MPa，断裂伸长率为320%，具有良好的生物相容性，相对细胞活力为99.6%，相对抗菌率分别为96.3%（大肠杆菌）和95.8%（金黄色葡萄球菌），在pH为5.0时的释放率为43.3%，15天时的伤口闭合率为90.5%，有助于改善伤口愈合性能，并为定制抗菌伤口敷料提供了新的可能性。
