School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China.
School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou 225127, China.
Food Chem. 2025 Jan 15;463(Pt 3):141395. doi: 10.1016/j.foodchem.2024.141395. Epub 2024 Sep 21.
Intake of 17β-estradiol (E2), bisphenol A (BPA), and diethylstilbestrol (DES) from food can contribute to endocrine disorders. Therefore, developing a sensitive method for the simultaneous detection of E2, BPA, and DES and understanding their combined effects on endocrine disruption are crucial. We developed a fluorescence aptasensing platform utilizing DNase I-assisted cyclic enzymatic signal amplification in conjunction with an aptamer/graphene oxide complex. Using PEG 20000 as a surface-blocking agent, the aptasensor achieved ultralow detection limits of 2.643, 0.3039, and 0.6996 for E2, BPA, and DES, respectively. The sensor demonstrated accurate detection in plastic bottled water at spiked levels of 10 and 100 ng/mL. Systems toxicology revealed 30 potential targets for mixture-induced endocrine disruption. Molecular docking showed binding affinities of E2, BPA, and DES for ESR1 of -9.94, -8.29, and - 8.98 kcal/mol, respectively. These results highlight the effectiveness of the aptasensor and provide valuable insights into endocrine disruption mechanisms.
从食物中摄入 17β-雌二醇（E2）、双酚 A（BPA）和己烯雌酚（DES）可能导致内分泌紊乱。因此，开发一种用于同时检测 E2、BPA 和 DES 的灵敏方法，并了解它们对内分泌干扰的联合作用至关重要。我们开发了一种荧光适体传感平台，利用 DNase I 辅助的循环酶信号放大与适体/氧化石墨烯复合物结合。通过使用 PEG 20000 作为表面封闭剂，该适体传感器对 E2、BPA 和 DES 的检测限分别达到了 2.643、0.3039 和 0.6996 ng/mL。该传感器在浓度为 10 和 100 ng/mL 的瓶装矿泉水中进行了准确的检测。系统毒理学揭示了 30 种潜在的混合物诱导内分泌干扰靶点。分子对接表明，E2、BPA 和 DES 与 ESR1 的结合亲和力分别为-9.94、-8.29 和-8.98 kcal/mol。这些结果突出了适体传感器的有效性，并为内分泌干扰机制提供了有价值的见解。
