Kiviranta I, Tammi M, Jurvelin J, Säämänen A M, Helminen H J
Histochemistry. 1985;83(4):303-6. doi: 10.1007/BF00684375.
Staining of articular cartilage by the periodic acid-Schiff (PAS) method was measured using microspectrophotometry. Standard PAS technique with 2 h oxidation produced a distinct Schiff reaction in the cartilage sections. The staining increased with depth of the articular cartilage demonstrating distribution of the glycoproteins. The modified PAS method included a second, longer periodic acid treatment, which made the uronic acid of glycosaminoglycans PAS-positive. The modified PAS method proved to be highly specific for chondroitin sulphate, which was determined from the samples with gas chromatography. A statistically significant correlation between the Schiff reactivity and galactosamine content of the sections was observed. It is concluded that for articular cartilage standard and modified PAS methods are useful procedures for demonstrating local changes of glycoproteins and chondroitin sulphate, respectively.
采用显微分光光度法测定经高碘酸 - 希夫（PAS）法染色的关节软骨。标准PAS技术经2小时氧化后，软骨切片产生明显的希夫反应。染色随关节软骨深度增加，表明糖蛋白的分布情况。改良PAS法包括第二次更长时间的高碘酸处理，使糖胺聚糖的糖醛酸呈PAS阳性。改良PAS法被证明对硫酸软骨素具有高度特异性，通过气相色谱法从样品中测定硫酸软骨素。观察到切片的希夫反应性与半乳糖胺含量之间存在统计学上的显著相关性。结论是，对于关节软骨，标准PAS法和改良PAS法分别是用于显示糖蛋白和硫酸软骨素局部变化的有用方法。
