Kiviranta I, Tammi M, Jurvelin J, Helminen H J
University of Kuopio, Department of Anatomy, Finland.
J Anat. 1987 Feb;150:265-76.
A recently developed microspectrophotometric method was used to measure local changes of thickness and glycosaminoglycan (GAG) content in articular cartilage. The intensity of the GAG stain was recorded from the superficial, intermediate, deep and calcified zones of articular cartilage. At each site of analysis, both the average stain concentration and the total stain content were determined. Of the 10 predefined locations of the Beagle knee (stifle) joint, the thickest cartilage was observed in the medial condyle of the tibia, which also possessed the highest GAG concentration. In the femur, the summits of the condyles had 33% thicker cartilage than the peripheral parts, while the GAG concentration was about equal in both of these regions. The thinnest cartilage and smallest GAG concentration was at the posterior end of the lateral condyle of the femur. The GAG content in the medial condyle and in the patellar surface of the femur was slightly greater than that in its lateral condyle.
一种最近开发的显微分光光度法被用于测量关节软骨厚度和糖胺聚糖（GAG）含量的局部变化。从关节软骨的表层、中层、深层和钙化层记录GAG染色的强度。在每个分析部位，均测定平均染色浓度和总染色含量。在比格犬膝关节的10个预定义位置中，观察到胫骨内侧髁的软骨最厚，其GAG浓度也最高。在股骨中，髁的顶部软骨比周边部分厚33%，而这两个区域的GAG浓度大致相等。最薄的软骨和最小的GAG浓度位于股骨外侧髁的后端。股骨内侧髁和髌面的GAG含量略高于其外侧髁。
