Pereira Lailah Horácio Sales, Alves Amanda do Carmo, Lopes Gabriela Francine Martins, da Silva Brenda Fernandes, Vieira Mariana Sousa, Lopes Débora de Oliveira, Ferreira Jaqueline Maria Siqueira, Lara Dos Santos Luciana
Universidade Federal de São João del-Rei (UFSJ), Laboratório de Biologia Molecular, Divinópolis, MG, Brazil; Universidade Federal de São João del-Rei (UFSJ), Laboratório de Microbiologia Médica, Divinópolis, MG, Brazil.
Universidade Federal de São João del-Rei (UFSJ), Laboratório de Biologia Molecular, Divinópolis, MG, Brazil.
Braz J Infect Dis. 2024 Nov-Dec;28(6):103873. doi: 10.1016/j.bjid.2024.103873. Epub 2024 Sep 25.
Dengue is a disease with a high-impact on public health worldwide. Many researches have focused on the cell receptors involved in its pathogenesis. The role of soluble isoforms of DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) receptor in the process of Dengue Virus (DENV) infection is not well understood. This work proposes to evaluate changes in the infection process of Immature Dendritic Cells (iDCs) by DENV in the presence of DC-SIGN recombinant soluble isoforms 8, 10, and 12. The recombinant isoforms were built by heterologous expression, the DENV-2 was multiplied in the Aedes albopictus C6/36 cells and quantified in BHK-21 cells, and the iDCs were produced from the THP-1 strain. Infection assays were performed in the presence of iDCs, DENV-2, and isoforms 8, 10, and 12 separately at 25, 50 and 100 ng/mL. The final viral load was estimated by qPCR and statistical analysis was performed by Kruskal-Wallis and ANOVA tests. The iDC profile was confirmed by increasing expression of CD11c, CD86, and CD209 surface markers and maintaining CD14 expression. Infection assays demonstrated a 23-fold increase in DENV viral load in the presence of isoforms 8 and 10 at 100 ng/mL compared to the viral control (p < 0.05), while isoform 12 did not alter the viral load. It was possible to conclude that at 100 ng/mL isoforms (8 and 10) can interact with DENV, increasing viral infection, and potentially acting as opsonins.
登革热是一种对全球公共卫生有重大影响的疾病。许多研究都集中在其发病机制中涉及的细胞受体上。树突状细胞特异性细胞间黏附分子-3抓取非整合素（DC-SIGN）受体的可溶性异构体在登革热病毒（DENV）感染过程中的作用尚未完全明确。这项研究旨在评估在存在DC-SIGN重组可溶性异构体8、10和12的情况下，未成熟树突状细胞（iDCs）被DENV感染过程中的变化。通过异源表达构建重组异构体，DENV-2在白纹伊蚊C6/36细胞中增殖并在BHK-21细胞中定量，iDCs由THP-1细胞系产生。分别在25、50和100 ng/mL的iDCs、DENV-2以及异构体8、10和12存在的情况下进行感染试验。通过qPCR估计最终病毒载量，并通过Kruskal-Wallis和方差分析进行统计分析。通过增加CD11c、CD86和CD209表面标志物的表达并维持CD14表达来确认iDCs的特征。感染试验表明，与病毒对照相比，在100 ng/mL的异构体8和10存在时，DENV病毒载量增加了23倍（p < 0.05），而异构体12未改变病毒载量。可以得出结论，在100 ng/mL时，异构体（8和10）可以与DENV相互作用，增加病毒感染，并可能作为调理素发挥作用。
